2013 Fiscal Year Final Research Report
Screening of the glycans regulating stemness and differentiation of hematopoietic stem cells
| Project/Area Number |
24659468
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Soka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUWA Takashi 創価大学, 工学部生命情報工学科, ポスドク (50570719)
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| Research Collaborator |
伊藤 和義 創価大学, 工学研究科生命情報工学専攻, 大学院生
吉池 健治 創価大学, 工学研究科生命情報工学専攻, 大学院生
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 造血幹細胞 / ニッチ / 糖鎖 / ショウジョウバエ |
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| Research Abstract |
Drosophila lymph gland is a hematopoietic organ, in which pluripotent stem cells proliferate and mature cells differentiate. The posterior signaling center functions as the hematopoietic niche that is essential for the maintenance of hematopoietic stem cells. Recently, the role of the signaling for the maintenance and differentiation of hematopoietic stem cells has been identified in Drosophila lymph gland. However, glycan functions are not well understood. Here we performed screening of the glycans that maintain and differentiate Drosophila hematopoietic stem cells. To screen, we use RNA interference based on GAL4-UAS system. The knock down of some of glycan-related genes in hematopoietic stem cells or in the posterior signaling center changed the hematopoietic stem cell population and the number of differentiating cells. It indicated that these glycans play important roles in regulation of hematopoietic stem cells.
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