2014 Fiscal Year Final Research Report
Search for new virulence factors in Aspergillus fumigatus: Molecular biological analysis of carbohydrate recognition proteins
Project/Area Number |
24659479
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Infectious disease medicine
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Research Institution | Chiba University |
Principal Investigator |
GONOI Tohru 千葉大学, 真菌医学研究センター, 教授 (30134365)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | アスペルギルス症 / アスペルギルス・フミガタス / 遺伝子破壊 / マウス感染モデル / 自然免疫 / 糖鎖結合タンパク |
Outline of Final Research Achievements |
Human pathogen Aspergillus fumigatus spreads their spores in the air, and infects host mainly from the respiratory system. Increased inflection cases by the fungi are brought by increases of immunocompromised patients caused by, e.g., AIDS, cancer, diabetes, as well as by increase of elderly people in recent years. For the purpose of exploring infection mechanisms and pathogenic factors involved, we made several gene disruption mutants of A. fumigatus strain. Among the mutants, Af2g12180 deficient mutant showed significantly enhanced pathogenicity in mouse infection model. In mice infected with Af2g12180 deficient mutant, liver expression of TLR4, TLR5, Dectin1, CARD9, CCL5, CCL19 mouse genes were significantly suppressed in comparison to mice infected with wild-type A, fumigatus strain. The results suggest Af2g12180 deficient mutant enhanced its pathogenicity by avoiding innate immunity of host.
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Free Research Field |
医真菌学
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