2013 Fiscal Year Final Research Report
Screening of novel host factors related to HCV lifecycle for developing useful drugs
| Project/Area Number |
24659480
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Infectious disease medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
ITO Masahiko 浜松医科大学, 医学部, 助教 (50385423)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | C型肝炎ウイルス / 生活環 / 感染・複製 / 粒子形成 / 宿主遺伝子 |
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| Research Abstract |
A lot of host factors related to infection, replication and release of the hepatitis C virus (HCV) have been reported. However, the whole aspect does not yet become clear. Therefore, using novel gene knockdown methed 'shRNAmir', I tried the exhaustive knockingdown of 35,000 host genes. In this period, I did construction of the required plasmid, establishment of stable cell line, comfirmation of plasmid expression. In addition, I do the exhaustive screening at present. This study allows the identification of the novel host factors of HCV and useful knowledgement for the elucidation of HCV life cycle as well as more effective treatment.
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