2014 Fiscal Year Final Research Report
Development of a method to evaluate the fatty acid oxidation impairment by infection using cultured cells and tandem mass spectrometry, in children
| Project/Area Number |
24659498
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 脂肪酸酸化 / セレウリド / タンデムマス法 / in vitro probe assay / 急性脳症 / 解熱剤 / heat stress |
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| Outline of Final Research Achievements |
The mechanism of acute encephalopathy in young children is often unknown in many cases. Onset of acute encephalopathy is not infrequently similar to that of congenital fatty acid oxidation (FAO) disorders. Influence of bacterial toxins, antipyretic drugs, or heat stress by hyperpyrexia on FAO was determined by in vitro probe (IVP) assay using cultured fibroblasts, and tandem mass spectrometry, changing the circumstance of cell culture.
The results were as follows: (i) cereulide, a toxin of Bacillus cereus, which occasionally causes acute encephalopathy in infants, disturbed FAO; (ii) heat stress possibly causes exacerbation of FAO in long-chain FAO disorders; (iii) antipyretics, salicylate (metabolites of Aspirin), and Diclofenac disturbed FAO in normal control cells, while acetaminophen did not. These findings may be consistent with clinical observation. The above findings will be of help for prevention of acute encephalopathy in children with infectious disease and/or hyperpyrexia.
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| Free Research Field |
小児科学、先天代謝異常
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