2014 Fiscal Year Final Research Report
Molecular mechanism responsible for psychiatric characteristics of PWS patients
| Project/Area Number |
24659505
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
|
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 転写後修飾 / snoRNA |
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| Outline of Final Research Achievements |
In order to modify MBII-52 snoRNA gene cluster on the genome of mouse ES cell, several attempt were made to insert two lox elements into the respective ends of the targeted region by use of a single targeting vector. However, no ES cell clone with expected recombination was obtained, probably because of the long distance (~100 kb) between these two sites, and it seems difficult to achieve desirable recombination on this gene cluster with the standard targeting strategy. Additional targeting experiment is currently ongoing using two vectors which interact independently with the two targeted sites. Consequent recombinant ES cells will be used for production of mice in which the number of MBII-52 gene repeat is manipulated, which are to be subjected to behavioral and biochemical analyses.
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| Free Research Field |
生物化学
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