2012 Fiscal Year Final Research Report
Relationship between acquired mutations of Th17 pathway related genes and onset of psoriasis
| Project/Area Number |
24659521
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Noriaki 千葉大学, 医学部附属病院, 講師 (00334186)
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| Project Period (FY) |
2012
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| Keywords | 乾癬 / Th17 |
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| Research Abstract |
We tried to verify the hypothesis that mutations of Th17pathway-related genes are responsible for the pathogenesis of psoriasis. Although thereare many inflammatory cells in patients skin, we failed to obtain sufficient number of cellsfrom isolated tissue. By subsequent immunofluorecence staining and FACS sorting, fewTh17 cells were recoverd and mutation analysis was impossible. We think that analysis ofcDNA synthesizied from RNA isolated from frozen tissue using new generation DNAsequencer enable us to identify mutations and still continue this study.
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