Cholesterol ointment suppressed hapten-induced dermatitis by inducing 11B-HSD1

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659529
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: KATAYAMA ICHIRO 大阪大学, 医学(系)研究科(研究院), 教授 (80191980)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: アトピー性皮膚炎 / 皮膚バリア / コレステロール / 脂質 / コルチゾール / 11β-HSD1 / 表皮角化細胞 / 保湿剤

Research Abstract

Changes in the stratum corneum extracellular matrix impair epidermal barrier function and may cause dermatoses in atopic dermatitis patients and elderly people. The aim of this study was to examine the effect of exogenous cholesterol application on skin barrier function and cutaneous inflammation. Hapten-stimulated mice were treated with topical cholesterol. The effect of topical cholesterol application on an oxazolone (OXA)-induced hypersensitivity reaction was evaluated. Topical application of cholesterol efficiently decreased transepidermal water loss in areas of barrier-disrupted skin and ameliorated OXA-induced cutaneous hypersensitivity. These favourable effects may have resulted from sustained expression of 11beta-hydroxysteroid dehydrogenase type 1 (11B-HSD1) in the cholesterol-treated skin. As 11B-HSD1 is known to produce active cortisol, topical cholesterol may attenuate contact hypersensitivity by normalizing secretion of hormonally active cortisol from the skin.

Research Products

• [Journal Article] 11β-hydroxysteroid dehydrogenase 1 specific inhibitor increased dermal collagen content and promotes fibroblast proliferatio (2014)
  • Author(s): Terao M, Tani M, Itoi S, Yoshimura T, Hamasaki T, Murota H, Katayama I.
  • Journal Title: PLoS One Volume: 9(3)
• [Journal Article] IFN-γor IFN-αAmeliorates Chronic Proliferative Dermatitis by Inducing Expression of Linear Ubiquitin Chain Assembly Complex (2014)
  • Author(s): Tamiya H, Terao M, Takiuchi T, Nakahara M, Sasaki Y, Katayama I, Yoshikawa H, Iwai K.
  • Journal Title: J Immunol Volume: 192(8) Pages: 3793-804
• [Journal Article] Topical cholesterol treatment ameliorates hapten-evoked cutaneous persensitivity by sustaining expression of 11β-HSD1 in epidermis (2014)
  • Author(s): Murota H, Itoi S, Terao M, Matsui S, Kawai H, Satou Y, Suda K, Katayama I.
  • Journal Title: Exp Dermatol Volume: 23(1) Pages: 68-70
• [Journal Article] Expression profiles of cortisol-inactivating enzyme, 11β-hydroxysteroid dehydrogenase-2, in human epidermal tumors and its role in keratinocyte proliferation (2013)
  • Author(s): Terao M, Itoi S, Murota H, Katayama I.
  • Journal Title: Exp Dermatol Volume: 22(2) Pages: 98-101
• [Journal Article] 11β-Hydroxysteroid dehydrogenase 1 contributes to the pro-inflammatory response of keratinocytes (2013)
  • Author(s): Itoi S, Terao M, Murota H, Katayama I.
  • Journal Title: Biochem Biophys Res Commun Volume: 440(2) Pages: 265-70
• [Journal Article] Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model (2013)
  • Author(s): Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S.
  • Journal Title: J Invest Dermatol Volume: 133(11) Pages: 2555-65
• [Presentation] Topical Vitamin D3 Therapy for Steroid Resistant Prurigo : Anti-inflammatory Action or Neuroprotective Effect (2013)
  • Author(s): Ichiro Katayama, Mika Terao, Mayuko Nakano, SJ Bae, Hiroyuki Murota
  • Organizer: 7th World Congress on ITCH
  • Place of Presentation: Boston, USA
  • Year and Date: 2013-09-21
• [Presentation] Upregulation of periostin by histamine via ERK1/2 pathway is essential for histamine-induced collagen production in dermal fibroblasts (2013)
  • Author(s): Linli Yang, Hiroyuki Murota, Saki Matsui, Mika Terao, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-09
• [Presentation] Oligosaccharide modification by N-acetylglucosaminyltransferase-V promotes skin sclerosis by inducing macrophages to shift toward M2 (2013)
  • Author(s): Mika Terao, Arisa Kato, Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-09
• [Presentation] Topical cholesterol application ameliorates hapten-evoked cutaneous hypersensitivity by sustaining expression of 11βHSD-1 in epidermis (2013)
  • Author(s): H Murota, M Terao, S Matsui, H Kawai, Y Satou, K Suda, I Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-08
• [Presentation] Expression profiles of cortisol inactivating enzyme, 11β-hydroxysteroid dehydrogenase 1 and 2 in human epidermal tumors and its role in keratinocyte proliferation (2013)
  • Author(s): Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: 1st Dermatoendocrinology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-07
• [Remarks]
  • URL: http://derma.med.osaka-u.ac.jp/