2012 Fiscal Year Final Research Report
Development of activator for skin function using bone marrow mesenchymal stem cell mobilizer
| Project/Area Number |
24659530
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Osaka University |
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Principal Investigator |
TAMAI Katsuto 大阪大学, 医学系研究科, 寄附講座教授 (20236730)
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| Project Period (FY) |
2012
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| Keywords | HMGB1 / 間葉系幹細胞 / 表皮水疱症 |
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| Research Abstract |
Using skin transplantation system on GFP-transgenic bone marrow-transplanted mouse, bone marrow-derived mesenchymal stem cells (MSCs) in the skin graft were evaluated by flow cytometry analysis. As a result, bone marrow -derived MSCs in the skin graft increased time dependently, and reached to ~1 % of all cell population in the skin graft at day 7 after the engraftment. Further precise investigation revealed that particular cell population (PDGFRα+/SSEA3+) in MSCs is a pluripotent stem cells harboringkeratinocyte-differentiation potential. Furthermore, intravenous administration of HMGB1, which had been shown to mobilize bone marrow MSCs into the circulation, showed potent activities to improve function of the skin graft by increasing MSC numbers, enhancing angiogenesis, and suppressing inflammation in the skin graft.
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Research Products
(14 results)
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[Journal Article] Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques.2013
Author(s)
Kotani M, Kikuta J, Klauschen F, Chino T, Kobayashi Y, Yasuda H, Tamai K, Miyawaki A, Kanagawa O, Tomura M, Ishii M
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Journal Title
J Immunol
Volume: 190(2)
Pages: 605-612
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[Journal Article] HIG1, a novel regulator of mitochondrial γ-secretase, maintains normal mitochondrial function2012
Author(s)
Hayashi H, Nakagami H, Takeichi M, Shimamura M, Koibuchi N, Oiki E, Sato N, Koriyama H, Mori M, Gerardo Araujo R, Maeda A, Morishita R, Tamai K, Kaneda Y
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Journal Title
FASEB J
Volume: 26(6)
Pages: 2306-2317
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[Journal Article] Two cases of recessive dystrophic epidermolysis bullosa diagnosed as severe generalized2012
Author(s)
Ohashi M, Shu E, Nagai M, Murase K, Nakano H, Tamai K, Sawamura D, Hiroka T, Seishima M, Kitajima Y, Aoyama Y
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Journal Title
J Dermatol
Volume: 38
Pages: 893-9
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