2014 Fiscal Year Final Research Report
A rapid, comprehensive system for assaying DNA repair activity and cytotoxic effects of DNA-damaging reagents
Project/Area Number |
24659533
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
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Research Institution | Nagasaki University |
Principal Investigator |
OGI Tomoo 長崎大学, 原爆後障害医療研究所, 准教授 (80508317)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | ヌクレオチド除去修復 |
Outline of Final Research Achievements |
Nucleotide excision repair (NER) is most versatile DNA repair process: it prevents cells from genomic instability as it removes various DNA damages arose in the genome. DNA repair assays that can directly measure NER activities are useful for clinical diagnoses and clinical researches including anticancer drug discovery. Unscheduled DNA synthesis (UDS) and recovery of RNA synthesis (RRS) are two commonly used DNA repair endpoints for assessing the activity of NER. We developed a comprehensive procedure for measuring UDS, RRS and cell-sensitivity by the incorporation of ethynyl-nucleoside analogs (EdU and EU) followed by the click-chemistry reaction. The developed system is suitable for a virus-based UDS / RRS / cell-sensitivity complementation tests, by which we can systematically determine the pathogenic gene of a patient with defect in the DNA repair pathways. A typical clinical diagnosis, using human primary fibroblast cells, takes less than 1 week.
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Free Research Field |
DNA修復・人類遺伝学・皮膚科学
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