2013 Fiscal Year Final Research Report
Comprehensive identification of the Pet-1-target genes by chromatin immunoprecipitation assays in RN46A cells derived from the rat raphe nucleus.
| Project/Area Number |
24659548
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
MATSUI Hiroaki 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (90181685)
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| Research Collaborator |
NAWA Yukino 聖マリアンナ医科大学, 医学(系)研究科(研究院), 助教 (10549786)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | セロトニン神経系 / クロマチン免疫沈降法 / ラット縫線核由来RN46A細胞 / セロトニン神経細胞特異的転写因子Pet-1 / Pet-1標的遺伝子 / 神経特異的抑制因子 |
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| Research Abstract |
To identify Pet-1 (serotonergic neuron-specific transcription factor)-binding sites in rat raphe nucleus-derived RN46A cells, chromatin-immunoprecipitation (ChIP) assays were performed. It was revealed that Pet-1 binding was inhibited through the neuron-restrictive silencer factor(NRSF)-NRSF binding element(NRSE) system or the potential repressor proteins independent of NRSF. Additionally, the detection of Pet-1 binding to target genes was occasionally difficult even under conditions where Pet-1 was overexpressed. Taken together, to exclude the function of repressor proteins including NRSF may be necessary to identify the Pet-1 binding to its target genes by ChIP assays in RN46A cells. To overcome these difficulties should be required to use RN46A cells as a proper model of central serotonergic neurons and characterize the Pet-1-mediated transcriptional network in serotonergic neurons.
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