2013 Fiscal Year Final Research Report
Invivo molecular mechanism and micro morphology of brain diseases in mice
| Project/Area Number |
24659578
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
KANNO Iwao 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, 主任研究員 (10360356)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | PET / 低酸素飼育マウス / TSPO発現 / インテグリン発現 / 11C-PK11195 / 64Cu-RAFT-RGD / オートラジオグラフィ / 免疫染色 |
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| Research Abstract |
We evaluated tissue pathophysiology and angiogenesis in the brain induced by hypoxia in mouse. B6 mice kept in 8-9% oxygen chamber for 0, 4, 7, 10, 14 days. Using PET, the TSPO emergence in the brain was estimated by employing[C11]PK11195 (PK), and the endothelium integrin was estimated by emplying [Cu64]RAFT-RDG (RDG). Autoradiography (ARG) was performed after RDG-PET. PK-PET revealed the SUV was peaked at 4th day, suggesting that microglia was maximally activated at 4th day. On the otherhand, RDG-PET was difficult to evaluate the maximum peak date of radioactivity in the brain due to too little entrance of the RDG through BBB. RDG-ARG revealed radioactivity in the brain parenchymal tissue only in the 14th day, suggesting angiogenesis was occurred at least 14th day, however, it is not clear whether angiogenesis appeared earlier than 14th day.
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