2013 Fiscal Year Final Research Report
generation of the HCV-infectable mouse-an animal model for inflammation cancer
| Project/Area Number |
24659585
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | C型肝炎ウイルス / 感染モデルマウス / ウイルス受容体 / ヒト感染因子 |
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| Research Abstract |
To investigate whether mouse Cd81, or Ocln inhibited the entry of HCV into mouse hepatocytes, we established the Cd81-expressing Huh7.5.1 cells and the Ocln-expressing Huh7.5.1 cells. HCVpp assay showed that no significant differences the infectivity of the virus among the parental, Cd81- and Ocln-Huh7.5.1 cells. This means that mouse Cd81 or mouse Ocln protein does not inhibit the entry process of HCV into mouse hepatocytes.
Next we planned to express the large amounts of human CD81, OCLN, CLDN1 and SCAVRB1 and made the targeting vectors to obtain the homologously recombinated ES cells. We obtained 4 ES clones for CD81/OCLN expression and one clone for CLDN1/SCAVRB1.
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