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2013 Fiscal Year Final Research Report

Development of artificial niche matrices for cardiac differentiation of stem cells

Research Project

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Project/Area Number 24659597
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

NAKATANI Takeshi  独立行政法人国立循環器病研究センター, 病院, 部長 (60155752)

Co-Investigator(Kenkyū-buntansha) YAMAOKA Tetsuji  独立行政法人国立循環器病研究センター, 研究所, 部長 (50243126)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords人工臓器学 / 心筋梗塞
Research Abstract

Stem cell-based myocardial regeneration has been emerged as alternative strategy for the heart transplantation or destination therapy using left ventricular assist system (LVAS). Especially self-beating cardiomyocytes are promising cells, but effective protocols to prepare the mature cardiomyocytes from various stem cells have not been clarified yet. The purpose of this article is find out the effect of stem cell culture niche (ECM proteins and substrate elasticities) on the cardiac differentiation, beating induction, and beating duration separately by using iPS cells (iPSs) and neonatal cardiomyocytes (NCMs). The cardiac differentiation step was greatly enhanced on Col-I-gel but the beating-induction step (a-MHC, TnC1, TnT2 expression) was effective on FN-culture dishes. The beating-duration step, which is assessed by counting the number of beating colonies of NCMs, preferred Col-I–gel. We also found the effective surface for keeping the undifferentiated stages of iPS cells.

  • Research Products

    (16 results)

All 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Book (5 results)

  • [Journal Article] Aortic valve closure for rapidly deteriorated aortic insufficiency after continuous flow left ventricular assist device implantation2013

    • Author(s)
      Fujita T, Kobayashi J, Hata H, Seguchi O, Sato T, Yanase M, Murata Y, Sunami H, Nakatani T
    • Journal Title

      J Artif Organs

      Volume: Vol.16 Pages: 98-100

    • DOI

      10.1007/s10047-012-0661-5

    • Peer Reviewed
  • [Journal Article] A large mobility of hydrophilic molecules at the outmost layer controls the protein adsorption and adhering behavior with the actin fiber orientation of human umbilical vein endothelial cells (HUVEC)2013

    • Author(s)
      Kakinoki S, Seo , JH, Inoue Y, Ishihara K, Yui N, Yamaoka T
    • Journal Title

      Journal of Biomaterials Science, Polymer Edition

      Volume: Vol.24, No.11 Pages: 1320-1332

    • DOI

      10.1080/09205063.2012.757726

    • Peer Reviewed
  • [Journal Article] Impact of ump replacement on outcome in advanced heart failure patients with left ventricular assist system2013

    • Author(s)
      Iwashima Y, Yanase M, Horio T, Seguchi O, Murata Y, Fujita T, Toda K, Kawano Y, Nakatani T
    • Journal Title

      Artificial Organs

      Volume: Vol.37, No. 7 Pages: 606-614

    • DOI

      10.1111/aor.12045

    • Peer Reviewed
  • [Journal Article] Off-pump coronary artery bypass grafting for a left main lesion due to cardiac allograft vasculopathy in Japan : first report of a case2013

    • Author(s)
      Fujita T, Kobayashi J, Hata H, Murata Y, Seguchi O, Yanase M, Shimahara Y, Sato S, Nakatani T
    • Journal Title

      Surg Today

    • DOI

      10.1007/s00595-013-0651-0

    • Peer Reviewed
  • [Journal Article] A heart transplant candidate with severe pulmonary hypertension and extremely high pulmonary vascular resistance2013

    • Author(s)
      Sato T, Seguchi O, Morikawa N, Hieda M, Watanabe T, Sunami H, Murata Y, Yanase M, Hata H, Fujita T, Nakatani T
    • Journal Title

      J Artif Organs

      Volume: Vol.16 Pages: 253-257

    • DOI

      10.1007/s10047-013-0695-3

    • Peer Reviewed
  • [Journal Article] Bone-destroying candida infection following left ventricular assist dvice explant2013

    • Author(s)
      Seguchi O, Fujita T, Murata Y, Yanase M, Higashi M, Toda K, Nakkatani T
    • Journal Title

      J Artif Organs

      Volume: Vol.16 Pages: 258-262

    • DOI

      10.1007/s10047-013-0696-2

    • Peer Reviewed
  • [Journal Article] 心臓移植および補助人工心臓の経験2013

    • Author(s)
      中谷武嗣、秦広樹、藤田知之、小林順二郎、村田欣洋、瀬口理、簗瀬正伸、堀由美子、和田恭一、植田初江、宮田茂樹、内藤博昭
    • Journal Title

      胸部外科

      Volume: Vol. 66、No. 1 Pages: 63-67

    • Peer Reviewed
  • [Journal Article] Serial changes in renal function as a prognostic indicator in advanced heart failure patients with left ventricular assist system2012

    • Author(s)
      Iwashima Y, Yanase M, Horio T, Seguchi O, Murata Y, Fujita T, Toda K, Kawano Y, Nakatani T
    • Journal Title

      Ann Thorac Surg

      Volume: Vol. 93 Pages: 816-824

    • DOI

      10.1016/j-athoracsur.2011.11.058

    • Peer Reviewed
  • [Journal Article] Impact of preoperative percutaneous cardiopulmonary support on outcome following left ventricular assist device implantation2012

    • Author(s)
      Toda K, Fujita T, Kobayashi J, Shimahara Y, Kitamura S, Seguchi O, Murata Y, Yanase M, Nakatani T
    • Journal Title

      Circulation Journal

      Volume: Vol. 76 Pages: 88-95

    • DOI

      10.1253/circj.CJ-11-0339

    • Peer Reviewed
  • [Journal Article] Risk factors for post-transpant low output syndrome2012

    • Author(s)
      Fujita T, Toda K, Yanase M, Seguchi O, Murata Y, Ishibashi-Ueda H, Kobayashi J and Nakatani T
    • Journal Title

      Eur. J Cardio-Thor Surg

      Volume: Vol. 42 Pages: 551-556

    • Peer Reviewed
  • [Journal Article] Paracorporeal ventricular assist device as a bridge to transplant candidacy in the era of implantable continuous-flow ventricular assist device

    • Author(s)
      Suwa H, Seguchi O, Fujita T, Murata Y, Hieda M, Watanabe T, Sato T, Sunami H, Yanase M, Hata H, Nakatani T
    • Journal Title

      J Artif Organs

      Volume: (in press)

    • DOI

      10.1007/s10047-013-0731-3

    • Peer Reviewed
  • [Book] 補助循環(IABP, PCPS)「今日の循環器疾患治療指針」2013

    • Author(s)
      中谷武嗣
    • Total Pages
      348-349
    • Publisher
      医学書院
  • [Book] 心不全における免疫吸着療法「今日の循環器疾患治療指針」2013

    • Author(s)
      中谷武嗣
    • Total Pages
      349-350
    • Publisher
      医学書院
  • [Book] 慢性心不全の非薬物療法4.補助人工心臓と心臓移植心臓移植の適応について教えてください、「心不全診療Q&Aエキスパート106人からの回答」2012

    • Author(s)
      中谷武嗣
    • Total Pages
      358-360
    • Publisher
      中外医学社
  • [Book] 慢性心不全の非薬物療法4.補助人工心臓と心臓移植心臓移植の手続きについて教えてください、「心不全診療Q&Aエキスパート106人からの回答」2012

    • Author(s)
      中谷武嗣
    • Total Pages
      361-363
    • Publisher
      中外医学社
  • [Book] ニプロVADシステム「心臓外科Knack & Pitfalls心不全外科治療の要点と盲点」2012

    • Author(s)
      中谷武嗣
    • Total Pages
      174-179
    • Publisher
      文光堂

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Published: 2015-06-25  

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