2013 Fiscal Year Final Research Report
Evaluation of liver injury and function by dynamic analysis of stresses
| Project/Area Number |
24659598
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
SATOU Naoki 北海道大学, 大学病院, 准教授 (70205946)
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| Co-Investigator(Kenkyū-buntansha) |
HAGA Sanae 北海道大学, 大学院保健科学研究院, 研究員 (60706505)
OZAKI Michitaka 北海道大学, 大学院保健科学研究院, 教授 (80256510)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 光プローブ / 小胞体ストレス / 細胞死 / ｐH / 生体イメージング |
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| Research Abstract |
We have developed 5 optic probes to evaluate liver stresses targeting intracellular molecular markers and pH. 1. a redox-sensitive mutant GFP (roGFP) probe for oxidative stress, 2. an optic probe for ER stress, 3. an optic probe for cell death (necroptosis), 4. an optic probe for cell viability (Akt phosphorylation), 5. an optic probe for cellular pH.
We have checked efficacy of these optic probes in liver cells. As for a roGFP probe and a pH-sensitive probe, we have checked usefulness of these probes in vivo, by using mice models.
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