Identification and characterization of hepatic cancer stem cells and cell biological investigation of oncologic process for developing new treatment of HCC

2012 Fiscal Year Final Research Report

• Project/Area Number: 24659606
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: UEMOTO Shinji 京都大学, 大学院・医学研究科・肝胆膵移植外科, 教授 (40252449)
• Co-Investigator(Kenkyū-buntansha): ISHII Takamichi 京都大学, 医学(系)研究科, 助教 (70456789)
• Project Period (FY): 2012
• Keywords: 再生医学 / 癌幹細胞 / 肝臓癌

Research Abstract

We investigated to the origin of CSCs and the oncogenic process of HCC. First, we successfully generated oncogene-regulatory expressing transgenic micewhich express the fusion protein of c-Myc and ER. We isolated fetal hepatic progenitor cells from the transgenic fetal mice by the method of cell aggregate which we established previously. And then, we isolated adult hepatocytes from adult transgenic mice. However, it is difficult for us to breed the transgenic mice, so we actually cannot use a needed amount of samples.We are trying to breed and increase the transgenic mice.After that, we will expose hepatocytes at each stage of differentiation to Tamoxifen and acquire cancer cells at several stages.

Research Products

• [Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage. (2012)
  • Author(s): Kamimura R, Ishii T, Sasaki N, Kajiwara M, Machimoto T, Saito M, Kohno K, Suemori H, Nakatsuji N, Ikai I, Yasuchika K, Uemoto S
  • Journal Title: Cell Transplant Volume: 21(11) Pages: 2351-62
  • DOI: DOI:10.3727/096368912X636957
• [Presentation] 大量肝切除後の急性肝不全に対する、肝細胞自家移植を用いた治療戦略 (2012)
  • Author(s): 喜多貞彦,石井隆道,小木曾 聡,片山外大,河合隆之,安田勝太郎,上村 良,梶原正俊,波多野悦朗,落合孝広,上本伸二
  • Organizer: 第39回日本臓器保存生物医学会
  • Place of Presentation: 福島
  • Year and Date: 2012-11-17
• [Presentation] 致死性肝障害モデルマウスに対する分化段階の異なる肝細胞移植の検討 (2012)
  • Author(s): 上村 良、石井 隆道、佐々木 直也、梶原 正俊、福光 剣,斉藤 美知子,河野 憲二,猪飼伊和夫,安近 健太郎,上本 伸二
  • Organizer: 第38回日本急性肝不全研究会
  • Place of Presentation: 金沢
  • Year and Date: 2012-06-06
• [Book] (2013)
  • Author(s): Takamichi Ishii, Kentaro Yasuchika, Iwao Ikai
  • Publisher: Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells