2013 Fiscal Year Final Research Report
Analysis of cancer stem cells related to metastasis and recurrence in hepatocellular carcinoma
| Project/Area Number |
24659610
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
OKA MASAAKI 山口大学, 医学(系)研究科(研究院), 教授 (70144946)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNEDOMI Ryouichi 山口大学, 大学院医学系研究科, 助教 (10420514)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 肝癌 / 癌幹細胞 |
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| Research Abstract |
The possible existence of plasticity between cancer stem cells and their more differentiated derivative cancer cells was suggested. We successfully induced cancer stem-like sphere cells from human HCC cell lines with specified medium. Poorly differentiated HCC derived SK-HEP-1 and undifferentiated HCC derived HLE cell lines efficiently formed spheres (SK-sphere and HLE-sphere). In the presence of several anti-cancer drugs except sorafenib, the cell viability of SK-sphere and HLE-sphere were significantly higher than that of parental cells. SK-sphere showed higher HIF1A mRNA expression, more CD44 variant-positive cells, and lower ROS production compared to parental cells. Integrated analysis showed that HIF1A and its downstream genes were up-regulated in SK-sphere. Finally, e identified several new marker and therapy target candidates (mRNA, microRNA, and protein) to cancer stem cells by integrated omics analysis.
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Research Products
(28 results)
