Comprehensive metabolome analysis of pancreatic cancer for identifying the pancreatic cancer-specific metabolic traits

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659612
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: YACHIDA Shinichi 独立行政法人国立がん研究センター, 研究所, ユニット長 (20359920)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 膵臓癌 / メタボローム

Research Abstract

The pancreatic cancer is a devastating disease. We have performed the comprehensive metabolome analysis method based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS), which allows quantitative analysis of thousands of charged metabolites. This approach was applied to simultaneous analysis of metabolite levels in tumor and normal counterparts obtained from 24 patients with pancreatic cancer. Enhanced glycolysis was found in tumor tissues, indicating the Warburg effect, which is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. In addition, quantification of hundred metabolites resulted in the identification of pancreatic cancer-specific metabolic traits. The amino acid concentrations were increased in tumor tissues. These data will apply into advanced imaging methods and blood tests to detect pancreatic cancer-specific metabolomes, which offer hope for such non-invasive early detection

Research Products

• [Journal Article] Heterogeneity of pancreatic cancer metastases in a single patient revealed by quantitative proteomics (2014)
  • Author(s): Kim MS, Zhong Y, Yachida S, Rajeshkumar NV, Abel ML, Marimuthu A, Mudgal K, Hruban RH, Poling JS, Tyner JW, Maitra A, Iacobuzio-Donahue CA, Pandey A
  • Journal Title: Mol Cell Proteomics Volume: (in press)(pii : mcp.M114.038547)
  • Peer Reviewed
• [Journal Article] Immunohistochemically detected expression of three major genes (CDKN2A/p16, TP53 and SMAD4/DPC4) strongly predicts survival in patients with pancreatic cancer (2013)
  • Author(s): Oshima M, Okano K, Muraki S, Haba R, Maeba T, Suzuki Y, Yachida S
  • Journal Title: Ann Surg Volume: 258 Pages: 336-346
  • DOI: 10.1097/SLA.0b013e3182827a6
  • Peer Reviewed
• [Journal Article] Iacobuzio-Donahue CA (2013)
  • Author(s): Yachida S
  • Journal Title: Oncogene Volume: 32 Pages: 5253-5260
  • DOI: 10.1038/onc.2013.29
  • Peer Reviewed
• [Journal Article] Clinicopathology of recurrent hepatocellular carcinomas after radiofrequency ablation treated with salvage surgery (2013)
  • Author(s): Yamamoto N, Okano K, Kushida Y, Deguchi A, Yachida S, Suzuki Y
  • Journal Title: Hepatol Res Volume: (Epub ahead of print)
  • DOI: 10.1111/hepr.12223
  • Peer Reviewed
• [Journal Article] Clinical significance of the genetic landscape of pancreatic cancer and implications for identification of potential long-term survivors (2012)
  • Author(s): Yachida S, White CM, Naito Y, Zhong Y, Brosnan JA, Macgregor-Das AM, Morgan RA, Saunders T, Laheru DA, Herman JM, Hruban RH, Klein AP, Jones S, Velculescu V, Wolfganag CL, Iacobuzio-Donahue CA
  • Journal Title: Clin Cancer Res Volume: 18 Pages: 6339-47
  • DOI: 10.1158/1078-0432.CCR-12-1215
  • Peer Reviewed
• [Journal Article] Novel therapeutic approaches in pancreatic cancer based on genomic alterations (2012)
  • Author(s): Yachida S
  • Journal Title: Curr Pharm Des Volume: 18 Pages: 2452-63
  • Peer Reviewed