2013 Fiscal Year Final Research Report
Possible interdependence between Warburg effect and genomic instability during colorectal cancer development
| Project/Area Number |
24659621
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所) |
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Principal Investigator |
SHIIBA KENITI 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん薬物療法研究部, 特任研究員 (90196345)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMA Hiroshi 地方独立行政法人宮城県立病院機構宮城 県立 がんセンター(研究所), がん薬物療 法研究部, 部長 (10196462)
TANUMA Nobuhiro 地方独立行政法人宮城県立病院機構宮城 県立 がんセンター(研究所), がん薬物療 法研究部, 主任研究員 (40333645)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 低酸素 / ワールブルグ効果 / DNA修復 / 大腸がん |
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| Research Abstract |
Novel mouse models to dissect isofom-specific functions of pyruvate kinase M (PKM) were established. Successful manipulations of PKM-switch in vivo were confirmed in colon and other organs/tissues of these mice. The mice were crossed to KrasG12D-LSL strain which conditionally express active mutant of Kras, and Pkm:Kras double Knock-in mice were established.
We also developed a novel mice strain on which Ppp6c gene is conditionally inactivated by treatment with 4-hydroxy tamoxifen. We began to cross the Ppp6c-mutant mice with aforementioned knock-in mice.
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