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2013 Fiscal Year Final Research Report

Establishment of a new strategy to improve graft function after lung transplantation from brain-dead donors using mesenchymal stem cells - preclinical study using MHC-inbred CLAWN miniature swine

Research Project

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Project/Area Number 24659637
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionKagoshima University

Principal Investigator

SAHARA Hisashi  鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 准教授 (90452333)

Co-Investigator(Kenkyū-buntansha) YAMADA Kazuhiko  鹿児島大学, 医用ミニブタ・先端医療開発 研究センター, 教授 (40241103)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywords移植・再生医療 / トランスレーショナルリサーチ / 肺移植 / 間葉系幹細胞 / 脳死 / 臓器保護 / ミニブタ
Research Abstract

Physiologic changes at brain death (BD) would affect the organ suitability and outcome of transplantation. We first examined whether donor BD affects early lung function and graft survival using MHC-defined CLAWN miniature swine. Up-regulation of proinflammatory cytokines as early as 6h after BD induction was observed in the BD donor associated with inflammatory cell infiltrates on 2-hour and 2-day biopsies. Moreover, graft survival using BD donor was significantly shorter compared to the non-BD donor (26 days vs 47 days). Next to establish a new strategy to reduce inflammatory change after brain death, we tried to establish porcine mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue, and successfully cultured them. After confirmation of the characteristics of these cells as MSC, we evaluate whether injection of MSC to brain-dead donor contribute to improve graft function and survival using preclinical lung transplant model of miniature swine.

  • Research Products

    (4 results)

All 2013 Other

All Presentation (3 results) Remarks (1 results)

  • [Presentation] Beneficial effects of inhaled carbon monoxide (CO) to brain-dead (BD) donors on prolonging pulmonary allograft survival in MHC-inbred CLAWN miniature swine2013

    • Author(s)
      Miura K, Sahara H, Waki S, Kawai A, Shimizu A, Yamada K
    • Organizer
      The 13th Congress of the Asian Society of Transplantation
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20130903-06
  • [Presentation] Carbon monoxide (CO) inhalation prolongs survival of the fully MHC-disparate lung graft from brain death donors in miniature swine2013

    • Author(s)
      Sahara H, Miura K, Kawai A, Waki S, Sekijima M, Shimizu A, Yamada K
    • Organizer
      International Society for Heart and Lung Transplantation 33rd Annual Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20130424-27
  • [Presentation] シンポジウム2「各系統実験用ブタ紹介」MHC確立クラウン系ミニブタを用いた同種移植実験2013

    • Author(s)
      佐原寿史、岩永健裕、山田和彦
    • Organizer
      第1回日本先進医工学ブタ研究会
    • Place of Presentation
      大阪市
    • Year and Date
      2013-11-12
  • [Remarks]

    • URL

      http://www.kufm.kagoshima-u.ac.jp/~xenotx/

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Published: 2015-06-25  

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