Potential novel treatment approach for cerebral aneurysm with mesenchymal stem cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659652
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Kyushu University
• Principal Investigator: NAKAMIZO AKIRA 九州大学, 医学(系)研究科(研究院), 講師 (80529800)
• Co-Investigator(Kenkyū-buntansha): HATA Nobuhiro 独立行政法人国立病院機構九州医療センター, 臨床研究センター (10596034) ; AMANO Toshiyuki 九州大学, 大学院医学研究院脳神経外科, 助教 (70448413) ; KIKKAWA Yuichiro 九州大学, 大学院医学研究院脳神経外科, 助教 (80423515)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 間葉系幹細胞 / 脳動脈瘤 / マクロファージ / micro RNA

Research Abstract

The macrophage is largely responsible for the development of cerebral aneurysms. We showed that macrophages secreted high level of matrix metalloproteinase (MMP)-9 via the intracellular and extracellular expression of microRNA-21. Moreover, we revealed that conditioned medium of mesenchymal stem cell suppressed the secretion of MMP-9 from macrophages. Furthermore, we showed that mesenchymal stem cells secreted a wide variety of microRNAs including microRNA-145 that inhibited the secretion of MMPs. This indicated that mesenchymal stem cell-derived exosomal microRNA-145 was introduced into macrophage, resulting in the suppression of MMP-9 secretion.