Mechanism of neruonal differention of pluripotent mesenchymal stem cells by intracullular delivery of BC-box motif peptides

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659655
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Yokohama City University
• Principal Investigator: KANNO Hiroshi 横浜市立大学, 医学(系)研究科(研究院), 客員准教授 (40244496)
• Co-Investigator(Kenkyū-buntansha): ITO Norihiko 鳥取大学, 農学部, 准教授 (80264654)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 多能性間葉系幹細胞 / BC-boxモチーフペプチド / 神経分化誘導

Outline of Final Research Achievements

In this study we used neural stem cells and skin-derived mesenchymal stem cells as pluripotent somatic stem cells to neuronal lineage.BC-box proteins-derived functional peptides correspoding to BC-box motif strucre[(A,P,S,T)LXXX (A,C) XXX(A,I,L,V)], showing induction activity of neuronal differentiation, are delivered to those somatic stem cells. Different BC-box motif peptides induced to differentiate different type of neuron, but two kinds of somatic stem cells differentiated to same neurons. This mechanism of neuronal differentiation is suggested to be related to blocking of binding between elongin BC & A, and inhibition of JAK2/Stat3, but is under investigation. From these results, it is considered that this study will contribute to neuronal regenerative medicine using somatic stem cells differentiated to useful neurons.

Free Research Field

脳神経外科学