Elucidation of biological functions of CD133, a marker for brain tumor-initiating cells, and its application

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659659
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: 愛知県がんセンター(研究所)
• Principal Investigator: IZAWA Ichiro 愛知県がんセンター(研究所), 腫瘍医化学部, 室長 (20311441)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 脳腫瘍 / がん幹細胞 / CD133

Research Abstract

CD133 is utilized as a marker for tumor-initiating cells of glioma, but its physiological function is still not well understood. To dissect the function of CD133, we tried to identify CD133-binding proteins, using pull-down methods, immunoprecipitation assays, and yeast two-hybrid techniques. On the other hand, we found that in human embryonic carcinoma NTERA-2 cells, CD133 suppresses the expression of Epithelial Splicing Regulatory Protein 1 (ESRP1), an RNA splicing factor that regulates epithelial-mesenchymal transition (EMT).

Research Products

• [Journal Article] Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells (2013)
  • Author(s): Matsuyama, M., Tanaka, H., Inoko, A., Goto, H., Yonemura, S., Kobori, K., Hayashi, Y., Kondo, E., Itohara, S., Izawa, I. and Inagaki, M
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 35626-35635
• [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γand is required for metaphase-anaphase transition (2013)
  • Author(s): Kasahara, K., Goto, H., Izawa, I., Kiyono, T., Watanabe, N., Elowe, S., Nigg, E.A. and Inagaki, M
  • Journal Title: Nat. Commun Volume: 4 Pages: 1882
  • DOI: 10.1038/ncomms2879
• [Presentation] Interaction of Cell Polarity Regulator Scribble with Multidrug Resistance Protein 4 (MRP4/ABCC4) (2013)
  • Author(s): Izawa, I
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2013-10-04
• [Presentation] 細胞極性制御因子ScribbleはMRP4/ABCC4と相互作用する (2013)
  • Author(s): 井澤一郎
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインク愛知(名古屋)
  • Year and Date: 2013-06-20
• [Presentation] LAP family protein Scribble interacts with Multidrug Resistance Protein 4 (MRP4/ABCC4) (2012)
  • Author(s): Izawa, I
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌)
  • Year and Date: 2012-09-20
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center