Function of ZNF449 in chondryocyte and mesenchymal tissues

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659664
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: NISHIDA Masahiro 東京大学, 医学部附属病院, 助教 (40622741)
• Co-Investigator(Kenkyū-buntansha): INUI Hiroshi 東京大学, 医学部附属病院, 助教 (60583119) ; SAITO Taku 東京大学, 医学部附属病院, 助教 (30456107)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 軟骨

Research Abstract

We carried out research on a new molecule named ZNF449 and focused on its function in chondrocytes. We started by investigating the expression pattern in the growth plate and found that it was expressed ubiquitously but mainly in the prehypertrophic region. In vitro experiments, such as promoter analysis and ATDC5 differentiation suggested involvement in the process of hypertrophy. Therefore, we created a knockout mouse and assessed its phenotype. Unfortunately, it did not show any growth retardation, nor did it show any difference when we created a surgically induced osteoarthritis model. We also analysed other mesenchymal tissues such as muscles and bones but we could not find any apparent difference between the wild-type mice.

Research Products

• [Journal Article] Application of cerebral oxygen saturation to prediction of the futility of resuscitation for out-of-hospital cardiopulmonary arrest patients : a single-center, prospective, observational study : Can cerebral regional oxygen saturation predict the futility of CPR? (2014)
  • Author(s): Fukuda T, Ohashi N, Nishida M, Gunshin M, Doi K, Matsubara T, Nakajima S, Yahagi N
  • Journal Title: Am J Emerg Med Volume: (Epub ahead of print) Pages: S0735-6757(14)00131-4
• [Journal Article] Recurrent hemarthrosis after total knee arthroplasty caused by the impingement of a remnant lateral meniscus : a case report (2014)
  • Author(s): Kawata M, Inui H, Taketomi S, Nakamura K, Nakagawa T, Tanaka S
  • Journal Title: Knee Volume: 21(2) Pages: 617-9
• [Journal Article] Identification of fibroblast growth factor-18 as a molecule to protect adult articular cartilage by gene expression profiling (2014)
  • Author(s): Mori Y, Saito T, Chang SH, Kobayashi H, Ladel CH, Guehring H, Chung UI, Kawaguchi H
  • Journal Title: J Biol Chem Volume: 289 Pages: 10192-200
• [Journal Article] Positive feedback between NF-κB and TNF-αpromotes leukemia-initiating cell capacity (2014)
  • Author(s): Kagoya Y, Yoshimi A, Kataoka K, Nakagawa M, Kumano K, Arai S, Kobayashi H, Saito T, Iwakura Y, Kurokawa M
  • Journal Title: J Clin Invest Volume: 124 Pages: 528-42
• [Journal Article] Clinical outcome of anatomic double-bundle ACL reconstruction and 3D CT model-based validation of femoral socket aperture position (2013)
  • Author(s): Taketomi S, Inui H, Nakamura K, Hirota J, Sanada T, Masuda H, Takeda H, Tanaka S, Nakagawa T
  • Journal Title: Knee Surg Sports Traumatol Arthrosc Volume: (Epub ahead of print)
• [Journal Article] Iliotibial band irritation caused by the EndoButton after anatomic double-bundle anterior cruciate ligament reconstruction : report of two cases (2013)
  • Author(s): Taketomi S, Inui H, Hirota J, Nakamura K, Sanada T, Masuda H, Tanaka S, Nakagawa T
  • Journal Title: Knee Volume: 20(4) Pages: 291-4
• [Journal Article] Influence of navigation system updates on total knee arthroplasty (2013)
  • Author(s): Inui H, Taketomi S, Nakamura K, Takei S, Takeda H, Tanaka S, Nakagawa T
  • Journal Title: BMC Sports Sci Med Rehabil Volume: 5(1) Pages: 10
• [Journal Article] An additional reference axis improves femoral rotation alignment in image-free computer navigation assisted total knee arthroplasty (2013)
  • Author(s): Inui H, Taketomi S, Nakamura K, Sanada T, Tanaka S, Nakagawa T
  • Journal Title: J Arthroplasty Volume: 28(5) Pages: 766-71
• [Journal Article] Generation of Col2a1-EGFP-iPS cells for monitoring chondrogenic differentiation (2013)
  • Author(s): Saito T, Yano F, Mori D, Ohba S, Hojo H, Otsu M, Eto K, Nakauchi H, Tanaka S, Chung UI, Kawaguchi H
  • Journal Title: PLoS ONE Volume: 8 Pages: e74137
• [Journal Article] Transcriptional induction of ADAMTS5 by an NF-κB family member RelA/p65 in chondrocytes during osteoarthritis development (2013)
  • Author(s): Kobayashi H, Hirata M, Saito T, Itoh S, Chung UI, Kawaguchi H
  • Journal Title: J Biol Chem Volume: 288 Pages: 28620-9
• [Journal Article] Cell-sheet technology combined with a thienoindazole derivative small compound TD-198946 for cartilage regeneration (2013)
  • Author(s): Yano F, Hojo H, Ohba S, Saito T, Honnami M, Mochizuki M, Takato T, Kawaguchi H, Chung UI
  • Journal Title: Biomaterials Volume: 34 Pages: 5581-7
• [Journal Article] Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development (2013)
  • Author(s): Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Pages: 1875-80
• [Journal Article] □-catenin Regulates PTH/PTHrP Receptor Signals and Chondrocyte Hypertrophy through Binding to Its Intracellular C-terminal Region (2013)
  • Author(s): Yano F, Saito T, Ogata N, Yamazawa T, Iino M, Chung UI, Kawaguchi H
  • Journal Title: Arthritis Rheum Volume: 65 Pages: 429-35
• [Journal Article] A novel disease-modifying osteoarthritis drug targeting Runx1 (2013)
  • Author(s): Yano F, Hojo H, Ohba S, Fukai A, Hosaka Y, Ikeda T, Saito T, Hirata M, Chikuda H, Takato T, Kawaguchi H, Chung UI
  • Journal Title: Ann Rheum Dis Volume: 72 Pages: 748-53