2013 Fiscal Year Final Research Report
Establishment of canine iPS cells for the study of disease-model dogs
| Project/Area Number |
24659675
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
IKEYA Makoto 京都大学, iPS細胞研究所, 准教授 (20442923)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Haruhisa 京都大学, iPS細胞研究所, 教授 (70332327)
MATSUI Hideto 奈良県立医科大学, 医学部, 講師 (00571027)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | iPS細胞 / イヌ |
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| Research Abstract |
To establish canine iPS cells, canine four reprogramming factor were introduced into canine embryonic fibroblasts with the EOS vector system, which allowed us to select clones with the expression of endogenous Oct3/4 gene by drug-resistance and fluorescence of GFP. Selected clones were positive for stem cell marker genes and able to make embryoid bodies, which showed the induction of marker genes specific for each of three germ layers. Teratoma formation was, however, not observed. Using chemically defined medium, these clones were maintained at undifferentiated state during several passages, but the long term culture forced the spontaneous differentiation of these clones, and therefore no clones with the feature compatible with iPS cells were established. These data indicated that more investigation will be required to find the suitable culture condition for canine iPS cells.
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Research Products
(20 results)
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[Journal Article] EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice2013
Author(s)
Yamada K, Ohno T, Aoki H, Semi K, Watanabe A, Moritake H, Shiozawa S, Kunisada T, Kobayashi Y, Toguchida T, Shimizu K, Hara A, Yamada Y
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Journal Title
J Clin Invest
Volume: 123(2)
Pages: 600-10
DOI
Peer Reviewed
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[Journal Article] Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin2013
Author(s)
Nasu A, Ikeya M, Yamamoto T, Watanabe A, Jin Y, Matsumoto Y, Hayakawa K, Amano N, Sato S, Osafune K, Aoyama T, Nakamura T, Kato T, Toguchida J
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Journal Title
PLos One
Volume: 8(1)
Pages: e53771
DOI
Peer Reviewed
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[Journal Article] Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation2013
Author(s)
Matsumoto Y, Hayashi Y, Schlieve CR, Ikeya M, Kim H, Nguyen TD, Sami S, Baba S, Barruet E, Nasu A, Asaka I, Otsuka T, Yamanaka S, Conklin BR, Toguchida J, Hsiao EC
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Journal Title
Orphanet J Rare Dis
Volume: 8(1)
Pages: 190
DOI
Peer Reviewed
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[Journal Article] Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells2012
Author(s)
Kajiwara M, Aoi T, Okita K, Takahashi R, Inoue H, Takayama N, Endo H, Eto K, Toguchida J, Uemoto S, Yamanaka S
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109(31)
Pages: 12538-43
DOI
Peer Reviewed
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