cartilage regeneration using decellularized tissue, peripheral blood mononuclear cell, and microRNA

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659677
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: OCHI Mitsuo 広島大学, 医歯薬保健学研究院(医), 教授 (70177244)
• Co-Investigator(Kenkyū-buntansha): MIYAKI Shigeru 広島大学, 病院, 講師 (10392490) ; KAMEI Naosuke 広島大学, 病院, 病院助教 (70444685) ; NAKASA Tomoyuki 広島大学, 病院, 病院助教 (60467769)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: microRNA / 変形性関節症 / エクソソーム / 脱細胞化 / 末梢血単核球

Research Abstract

The purpose of this study is to create cartilage tissue using decellularized cartilage, peripheral blood mononuclear cell and microRNA. It was impossible to induce chondrogenesis from peripheral blood mononuclear cell because the pellet culture of peripheral blood mononuclear cell could not be achieved. Osteochondral plug of rat was decellularized by 2%SDS for 12 hours, and decellularized osteochondral plug with cartilage matrix was obtained. But DNA content was not completely removed. Next, microRNA expression in the culture medium of chondrocyte sheet was analyzed. It was revealed that chondrocyte sheet secreted microRNAs which play a crucial role in chondrogenesis and osteogenesis.