2013 Fiscal Year Final Research Report

Development of novel methods for imaging of bone marrow utilizing fluorescence techniques and spatiotemporal analysis of bone metastasis

Research Project

Project/Area Number	24659679
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Orthopaedic surgery
Research Institution	Ehime University
Principal Investigator	HIKITA Atsuhiko 愛媛大学, 医学(系)研究科(研究院), 准教授 (60443397)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	骨・軟部腫瘍学
Research Abstract	To elucidate the molecular mechanisms of interaction between cancer cells and microenvironments which determine the osteolytic/ osteogenic tendencies of bone metastases of cancer, series of experiments were performed to establish an in vivo imaging system by which 4 kind of cells, cancer cells, osteoclasts, osteoblasts and osteocytes in bone marrow can be analyzed simultaneously at multiple time points. Cancer cells expressing fluorescent proteins were implanted to mice and bone metastasis system of osteolytic prostate cancer was established. In vivo fluorescence imaging for osteoclast was also performed.

(4 results)