2013 Fiscal Year Final Research Report
Analysis of the TGF-b signaling in cartilage by nonlinear optics imaging combined with genetic engineering techniques
| Project/Area Number |
24659680
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University |
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Principal Investigator |
MIURA Hiromasa 愛媛大学, 医学(系)研究科(研究院), 教授 (10239189)
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| Co-Investigator(Renkei-kenkyūsha) |
IMAMURA Takeshi 愛媛大学, 大学院医学系研究科, 教授 (70264421)
HIKITA Atsuhiko 愛媛大学, 大学院医学系研究科, 准教授 (60443397)
IIMURA Tadahiro 愛媛大学, 大学院医学系研究科, 准教授 (20282775)
OSHIMA Yusuke 愛媛大学, 医学部附属病院, 助教 (10586639)
MIYAZAKI Tsuyoshi 東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究員 (50376480)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | TGF-β / 軟骨 / 軟骨変性 / 変形性関節症 / SHG / 2光子励起 / Smurf |
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| Research Abstract |
Multi-photon microscopy enables to characterize and evaluate alternation of collagen matrix in vivo and in vitro based on second harmonic generation (SHG) signal. We performed SHG imaging of the cartilage degeneration induced by enzyme treatment. In the result, changes in SHG signal intensity were observed in the experimental samples. We also established an animal osteoarthritis model by syndesmectomy using H2B-GFP line mice. We successfully evaluated over time the changes in cartilage matrix and chondrocytes by SHG and two-photon excited fluorescence. Furthermore, the articular cartilages of aged mice were observed as the same manner. In comparison with those of young mice, it was possible to evaluate them as spontaneous knee osteoarthritis. Our technique that combined genetic engineering and nonlinear interaction between the molecules and photons to approach the mechanism of cartilage degeneration is promising as a translational research leading to clinical application.
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