In search for shared regulatory mechanisims of bone mineralization in vertebrates

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659685
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: MATSUO Koichi 慶應義塾大学, 医学部, 教授 (40229422)
• Co-Investigator(Kenkyū-buntansha): TAKADA Yasunari 慶應義塾大学, 医学部, 講師 (40407086)
• Research Collaborator: MATSUMOTO Tomohiro 東海大学, 医学部, 学生
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 骨代謝 / 石灰化 / 転写因子 / メダカ / 骨芽細胞 / ミネラリゼーション

Research Abstract

We generated transgenic medaka fish expressing mouse Fra-1 in osteoblasts to determine cell-autonomous function of Fra-1 in osteoblasts in vivo. Alizarin complexone staining and micro-computed tomography revealed that Fra-1 transgenic medaka showed lower bone mineral density than wild-type controls. These data suggest that Fra-1 expression in osteoblasts impairs bone mineralization not only in mice but also in medaka.

Research Products

• [Journal Article] 石灰化機序とその調節機構Mechanism and regulation of bone mineralization (2013)
  • Author(s): 松尾光一
  • Journal Title: Clin. Calcium Volume: 23 (10)(CliCa131014631467) Pages: 1463-1467
• [Presentation] Pericapillary Bone Formation by Mural Osteoblasts during Endochondral Ossification (2013)
  • Author(s): Ichiro Takada, Nobuhito Nango, Yoshihiro Takeda, Atsushi Momose, Yoshiaki Kubota, Sho Kanzaki, Kouji Shimoda, Yasunari Takada, Latifa Bakiri, Erwin Wagner and Koichi Matsuo
  • Organizer: 35th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Baltimore, Maryland, USA(Poster)
  • Year and Date: 20131004-07
• [Presentation] Impaired Bone Mineralization In Transgenic Medaka Fish Expressing Fra-1 In Osteoblasts (2013)
  • Author(s): Matsumoto, T., Takada, Y., Nakamura, T., Suematsu, M., Inohaya, K., Kudo, A., Shimizu, A. and Matsuo, K
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: 神戸
  • Year and Date: 20130528-0601
• [Presentation] バイオミネラリゼーション : 細胞からマルチスケールへ (2013)
  • Author(s): 松尾光一
  • Organizer: 第12回松本ボーンフォーラム
  • Place of Presentation: 長野
  • Year and Date: 20130517-18
  • Invited
• [Presentation] Transgenic expression of mouse Fra-1 in osteoblasts reduces bone mineralization in medaka fish (2012)
  • Author(s): Matsumoto, T., Takada, Y., Nakamura, T., Suematsu, M., Inohaya, K., Kudo, A., Shimizu, A., and Matsuo, K
  • Organizer: 小型魚類研究会(第18回)
  • Place of Presentation: 京都(英語口演)
  • Year and Date: 20120922-23
• [Book] 破骨細胞分化と活性化メカニズム。骨研究最前線～代謝・疾病のメカニズムから再生医療・創薬・リハビリ機器・機能性食品開発まで～ (2013)
  • Author(s): 松尾光一
  • Total Pages: 29-35
  • Publisher: (株)エヌ・ティー・エス