2013 Fiscal Year Final Research Report
Study of the expression and function of C2GnT1 in endometrial carcinoma
| Project/Area Number |
24659724
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shinshu University |
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Principal Investigator |
SUZUKI Akihisa 信州大学, 医学部附属病院, 特任研究員 (10547095)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOZAWA Tanri 信州大学, 医学部, 教授 (20235493)
MIYAMOTO Tsutomu 信州大学, 医学部付属病院, 講師 (70418721)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | C2GnT1 / 子宮内膜癌 / 予後因子 / コア 2 分枝糖鎖 |
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| Research Abstract |
Anti-human C2GnT1 rabbit polyclonal antibody were supplied from Prof. Nakayama
(Shinshu Univ. Molecular Pathology) and confirmed its specificity by western blotting and immunofluorescent staining using COS7 cells transfected human C2GnT1 cDNA. Using this anti-C2GnT1 antibody, the expression of C2GnT1 was examined in normal endometrium, atypical endometrial hyperplasia and endometrial carcinoma, immunohistochemically. The expression of C2GnT1 was significantly stronger in endometrial carcinoma than that in normal endometrium (P<0.05). In endometrial carcinomas, the stronger expression of C2GnT1 seemed to be involved in aggressiveness including higher histological grade, deep
myometrial invasion, and lymph-vascular space invasion. Kaplan-Meier method revealed that the cases with C2GnT1 overexpression were associated with shorter survival periods (P<0.05). In addition, multi-variable analysis revealed that C2GnT1 overexpression was an independent prognostic factor.
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