2014 Fiscal Year Final Research Report
Translational research aiming at drug development to enhance sperm motility, and to improve the fertility rate.
| Project/Area Number |
24659726
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANAYAMA Naohiro 浜松医科大学, 医学部, 教授 (70204550)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 生殖医学 / トランスレーショナル・リサーチ |
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| Outline of Final Research Achievements |
GWRQ enhanced motility of spermatozoa from wild type but not trophinin null mice. CASA showed that GWRQ enhanced both progressive motility and rapid motility in wild type mouse sperm.
FGF and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with EBG, which specifically polyLacs, enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.
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| Free Research Field |
生殖医療、婦人科腫瘍
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