2013 Fiscal Year Final Research Report
The role of fatty acid signaling in the cochlea
| Project/Area Number |
24659744
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Tohoku University |
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Principal Investigator |
TAKATA YUSUKE 東北大学, 医学(系)研究科(研究院), 非常勤講師 (60623999)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脂肪酸結合タンパク質 / Fabp / 蝸牛 / 難聴 / 脂肪酸 |
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| Research Abstract |
The fatty acid-binding proteins (Fabps) bind to fatty acids and mediate their uptake and transport, thereby regulating metabolic pathways and gene expression. In the mouse cochlea, Fabp3 and Fabp7 have been identified. However, the role of these Fabps remains unknown. Wild-type, Fabp3 KO, and Fabp7 KO mice on the C57BL/6 background were used. Auditory thresholds were measured by the ABR. Cochlear morphology was assessed via HE staining, immunostaining, and TEM. Transcriptome analysis was also performed. Fabp3 (-/-) mice exhibited no auditory phenotypes. In contrast, the ABR thresholds of Fabp7 (-/-) mice were significantly reduced compared to Fabp7 (+/+) mice at 12 months. The numbers of SG neurons and fibrocytes were remarkably preserved in Fabp7 (-/-) mice at 12 and 15 months. Transcriptome analysis indicated that Fabp7 deficiency caused up-regulation of carbohydrate metabolism-related genes and conversely caused down-regulation of lipid metabolism-related genes.
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[Presentation] Fatty Acid-Binding Protein 7 Deficiency Slows the Progression of Age-Related Hearing Loss in Mice by Modulating Metabolic Pathways2014
Author(s)
Suzuki J, Oshima T, Kimura R, Yoshida N, Yoshizaki K, Takata Y, Owada Y, Kawase T, Kobayashi T, Katori Y, Osumi N
Organizer
37th ARO MidWinter Meeting
Place of Presentation
San Diego,USA
Year and Date
20140222-26
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