2013 Fiscal Year Final Research Report

New analysis of inner ear pharmacology

Research Project

Project/Area Number	24659752
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Keio University
Principal Investigator	OGAWA Kaoru 慶應義塾大学, 医学部, 教授 (00169179)
Co-Investigator(Kenkyū-buntansha)	KANZAKI Sho 慶應義塾大学, 医学部, 講師 (50286556) FUJIOKA Masato 慶應義塾大学, 医学部, 共同研究院 (70398626)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	内耳 / 薬物動態
Research Abstract	Methods 1)Luciferin delivered into the inner ears of GFAP-Luc transgenic mice reacted with luciferase in GFAP-expressing cells in the cochlear spiral ganglion, resulting in photon bioluminescence.Both luciferin and hyaluronic acid (HA) were injected through the left side of ear. We changed the concentration and viscosity of HA. Signals were obtained in the left side of ear in GFAP-luc mice through the IVIS system. 2) Strong intensity noise exposed into male Guinea pigs for two hours. Combination of dexamethasone and HA was administrated into the ear.Results 1) Signal does not depend on the viscosity of hyaluronic acid of inner ear. One reason is that high viscosity of HA can not go through the round window membrane. We considered that the technical problem was involved.2)The question is "Is the combination of steroid and HA successful or not?"ABR was tested before and after treatment.

(3 results)