Atopic keratoconjunctivitis and natural helper cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659768
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Juntendo University
• Principal Investigator: MURAKAMI Akira 順天堂大学, 医学部, 教授 (90157743)
• Co-Investigator(Kenkyū-buntansha): EBIHARA Nobuyuki 順天堂大学, 医学部, 教授 (20255699) ; MATSUDA Akira 順天堂大学, 医学部, 准教授 (00312348)
• Co-Investigator(Renkei-kenkyūsha): NAKAE Susumu 東京大学, 医科学研究所, 准教授 (60450409)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: アトピー性角結膜炎 / ナチュラルヘルパー細胞 / IL-33 / 自然免疫 / パパイン

Research Abstract

We made innate immune system-dependent mouse conjunctivitis model using papain-absorbed contact lens. The lens was inserted into the conjunctival sac of B6 wild and B6 Rag2-kockout (KO) and IL-33 KO mice. In this mouse models, prominent eosinophilic infiltration was observed in the conjunctival tissue. The number of infiltrated eosinophils was significantly smaller in IL-33 KO mice compared to wild-type and Rag2-KO mice. These results suggested that IL-33 expression is essential for ocular surface allergic inflammatory process through the activation of innate immune systems. In addition, we detected natural helper (NH) cells in the lacrimal gland and conjunctival tissue of the innate immune system-dependent mouse conjunctivitis model. Moreover, we identified NH cell in the giant papillae tissue obtained from severe chronic allergic conjunctivitis patients. These results suggested that NH cell may participate the pathophysiological process of severe chronic allergic conjunctivitis.

Research Products

• [Journal Article] Vernal keratoconjunctivitis with giant papillae on the inferior tarsal conjunctiva (2014)
  • Author(s): Asada Y, Ebihara N, Funaki T, Norihiko Y, Murakami A, Matsuda A
  • Journal Title: Cornea Volume: 33 Pages: 32-34
  • Peer Reviewed
• [Journal Article] Interaction between conjunctival epithelial cells and mast cells induces CCL2 expression and piecemeal degranulation in mast cells (2013)
  • Author(s): Iwamoto S, Asada Y, Ebihara N, Hori K, Okayama Y, Kashiwakura JI, Watanabe Y, Kawasaki S, Yokoi N, Inatomi T, Shinomiya K, Murakami A, Matsuda A
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54 Pages: 2465-2473
  • Peer Reviewed
• [Presentation] Interleukin(IL)-33 and Thymic Stromal Lymphopoietin (TSLP), but not IL-25, induced Allergic Conjunctivitis in Mouse Models Papain-Induced Conjunctivitis (2014)
  • Author(s): Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae
  • Organizer: The annual meeting for the Association of Research in Vision and Ophthalmology
  • Place of Presentation: 米国オーランド
  • Year and Date: 20140500
• [Presentation] 中江進パパイン誘発結膜炎モデルマウスとナチュラルヘルパー細胞 (2013)
  • Author(s): 浅田洋輔、松田彰、堀寛爾、岩本怜、海老原伸行、村上晶
  • Organizer: 日本眼科学会総会
  • Year and Date: 20130400
• [Presentation] Detection of natural helper cells in innate immune system-dependent mouse conjunctivitis model (2013)
  • Author(s): Yosuke Asada, Akira Matsuda, Kanji Hori, Satoshi Iwamoto, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae
  • Organizer: The annual meeting for the Association of Research in Vision and Ophthalmology
  • Place of Presentation: 米国シアトル
  • Year and Date: 20130400
• [Presentation] The role of interleukin-33 (IL-33) in mouse experimental allergic conjunctivitis (2013)
  • Author(s): Akira Matsuda, Kanji Hori, Waka Ishida, Ken Fukuda, Nobuyuki Ebihara, Yousuke Asada, Susumu Nakae, Atsuki Fukushima, Akira Murakami. Keystone Symposia
  • Organizer: Type 2 Immunity : Initiation , maintainance, homeostasis and Pathology
  • Place of Presentation: 米国サンタフェ
  • Year and Date: 20130100