Effect of Umbilical Cord Mesenchymal Stem Cells On The Growth Of Nitrofen Induces Hypoplastic Lung Explants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659772
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: University of Tsukuba
• Principal Investigator: SHINKAI Toko 筑波大学, 医学医療系, 講師 (80301612)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 再生医療 / 幹細胞 / 臍帯由来間葉系幹細胞 / 横隔膜ヘルニア / 肺低形成

Outline of Final Research Achievements

Adipose stem cells have been reported to accelerate alveolar and vascular regeneration in damaged rat lungs. Therefore, we are investigating the possibility of regeneration therapy using human umbilical cord mesenchymal stem cells in an experimental hypoplastic lung models.
We used stem cells (hMSC-UC) which are commercially available from PromoCell. １．I used P2 passage culture cells. I created hMSC-UC sheet in 1cm diameter for 5 days culture.２．The ratio of the terminal bud increments of cultured lung was calculated. VEGF, BMP-4, TTF-1 mRNA expression levels were analyzed by RT-qPCR. Terminal lung buds significantly proliferated in embryonic lungs when co-cultured with hMSC-UC. VEGF, BMP-4, and TTF-1 mRNA expressions were all significantly higher in normal embryonic lungs when co-cultured with hMSC-UC. The study showed that hMSC-UC may stimulate secretion of lung growth factors such as; VEGF, BMP-4, and TTF-1 from the embryonic lung.

Free Research Field

小児外科