2013 Fiscal Year Final Research Report
Elucidation of the in vivo migration of mesenchymal stem cells.
| Project/Area Number |
24659789
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Tokyo Medical University (2013)
Keio University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 間葉系幹細胞 / 創傷治癒学 |
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| Research Abstract |
We report an improved prospective clonal isolation technique and reveal that the combination of three cell-surface markers (LNGFR, THY-1, and VCAM-1) allows for the selection of highly enriched clonogenic cells (one out of three isolated cells). Clonal characterization of LNGFR+THY-1+ cells demonstrated cellular heterogeneity among the clones. Rapidly expanding clones (RECs) exhibited robust multilineage differentiation and self-renewal potency, whereas the other clones tended to acquire cellular senescence via P16INK4a and exhibited frequent genomic errors. Furthermore, RECs exhibited unique expression of VCAM-1 and higher cellular motility compared with the other clones. The combination marker LNGFR+THY-1+VCAM-1hi+ (LTV) can be used selectively to isolate the most potent and genetically stable MSCs.
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Research Products
(18 results)
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[Journal Article] Homeodomain transcription factor meis1 is a critical regulator of adult bone marrow hematopoiesis2014
Author(s)
Ariki R, Morikawa S, Mabuchi Y, Suzuki S, Nakatake M, Yoshioka K, Hidano S, Nakauchi H, Matsuzaki Y, Nakamura T, Goitsuka R
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Journal Title
PLoS One
Volume: 9(2)
Pages: 87646
DOI
Peer Reviewed
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[Journal Article] Mesenchymal stem cells regulate epithelial-mesenchymal transition and tumor progression of pancreatic cancer cells2013
Author(s)
Kabashima-Niibe A, Higuchi H, Takaishi H, Masugi Y, Matsuzaki Y, Mabuchi Y, Funakoshi S, Adachi M, Hamamoto Y, Kawachi S, Aiura K, Kitagawa Y, Sakamoto M, Hibi T
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Journal Title
Cancer Sci
Volume: 104(2)
Pages: 157-64
DOI
Peer Reviewed
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