2013 Fiscal Year Final Research Report

Analysis of pathogenisis of metal allergy using humanized murine model

Research Project

Project/Area Number	24659809
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Morphological basic dentistry
Research Institution	Tohoku University
Principal Investigator	SUGAWARA Shunji 東北大学, 歯学研究科(研究院), 教授 (10241639)
Co-Investigator(Kenkyū-buntansha)	KUROISHI Toshinobu 東北大学, 大学院歯学研究科, 助教 (30400261) ENDO Yasuo 東北大学, 大学院歯学研究科, 技術補佐員 (50005039)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	金属アレルギー / ヒト化マウス / ニッケル
Research Abstract	The aim of this study was to elucidate pathogenesis of metal allergy using humanized mice by reconstituting human immune system in the mice. We used genetically modified immunodeficient mice as recipients and tried to engraft human peripheral blood mononuclear cells in the mice. We also depleted macrophages in the mice to increase immunodeficiency. We observed very weak engraftment of human lymphocytes in the mice but are unable to induce metal allergy. As analysis of pathogenesis of metal allergy using humanized mice is still fascinating project to develop medical service for the next generation, we continue to realize this project.