Development of novel therapeutic approach of rheumatoid arthritis with liposomalized lactoferrin

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659814
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: TAKATA Takashi 広島大学, 医歯薬保健学研究院(歯), 教授 (10154783)
• Co-Investigator(Kenkyū-buntansha): MIYAUCHI Mutsumi 広島大学, 医歯薬保健学研究院(歯), 准教授 (50169265) ; KITAGAWA Masae 広島大学, 大学病院, 助教 (10403627) ; INUBUSHI Toshihiro 広島大学, 医歯薬保健学研究院(歯), 助教 (30550941)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ラクトフェリン / 関節リウマチ / 骨破壊 / 炎症性サイトカイン / リポソーム

Research Abstract

Aim of the study was to clarify the effectiveness of liposomalized lactoferrin (L-Lf) as a new therapeutic agent of rheumatoid arthritis (RA). The following results were obtained.
1. Administration of L-Lf significantly reduced the joint destruction observed in RA model mice. 2. Lf inhibited expression of inflammatory cytokines induced in human macrophages and synovial cells by TNF-a. Through inhibition of the map kinase and NFkb pathways 3. By oral administration of L-Lf decreased Th17 and increased Treg populations, and reduced IL-17 level in serum.
These results show that l-lf could be used as a new therapeutic agent for RA.

Research Products

• [Journal Article] Lactoferrin inhibits infection-related osteoclastogenesis without interrupting compressive force-related osteoclastogenesis (2014)
  • Author(s): Inubushi T, Kawazoe A, Miyauchi M, Yanagisawa S, Subarnbhesaj A, Chanbora C, Ayuningtyas NF, Ishikado A, TanakK, Takata Ta E, Takata T
  • Journal Title: Arch Oral Biol Volume: 59(2) Pages: 226-32
  • DOI: 10.1016/j.archoralbio.2013.11.002
  • Peer Reviewed
• [Journal Article] Orally adoministrated liposomal lactoferrin inhibits inflammation-related bone brakedown with out interlapting orthodontic tooth movement (2013)
  • Author(s): Kawazoe A, Inubushi T, Miyauchi M, Ishikado A, Tanaka E, Tanne K, Takata T
  • Journal Title: J Periodontol Volume: 84(10) Pages: 1454-1462
  • DOI: 10.1902/jop.2012.120508
  • Peer Reviewed
• [Presentation] Oral administration of liposomalized lactoferrin inhibits bone resorption by periodontitis and other bone destructive diseases (2013)
  • Author(s): Takata T
  • Organizer: 8th International Dental Collaboration of the Mekong River Region Congress
  • Place of Presentation: プノンペン、カンボジア
  • Year and Date: 20131205-06
  • Invited
• [Presentation] Bovine lactoferrin enhances osteogenesis through TGF-βreceptor signaling (2013)
  • Author(s): Inubushi T, Kosai A, Yanagisawa S, Chanbora C, Miyauchi M, Yamasaki S, Sugiyama E, Ishikado A, Makino T, Takata T
  • Organizer: International Association of Dental Research- Asia Pacific Region
  • Place of Presentation: バンコク、タイ
  • Year and Date: 20131006-10
• [Presentation] Oral administration of liposomal bovine lactoferrin is useful for prevention and control of periodontitis (2013)
  • Author(s): Takata T, Inubushi T, Miyauchi M, Ishikado A, Makino T
  • Organizer: XIth International Conference on Lactoferrin : Structure, Function and Applications
  • Place of Presentation: ローマ、イタリア
  • Year and Date: 20131006-10
  • Invited
• [Presentation] Bovine lactoferrin enhances osteogenesis through TGF- beta receptor signaling (2013)
  • Author(s): Inubushi T, Kosai A, Yanagisawa S, Chanbora C, Miyauchi M, Yamasaki S, Sugiyama E, Ishikado A, Makino T, Takata T
  • Organizer: XIth International Conference on Lactoferrin : Structure, Function and Applications
  • Place of Presentation: ローマ、イタリア
  • Year and Date: 20131006-10
• [Presentation] ラクトフェリンによる骨吸収抑制作用とその分子生物学的メカニズム (2013)
  • Author(s): 犬伏俊博、宮内睦美、敖敏、石角篤、牧野武利、高田隆
  • Organizer: 第56回春季歯周病学会
  • Place of Presentation: 東京
  • Year and Date: 20130531-0601
• [Presentation] ラクトフェリンによる骨吸収抑制作用とその分子生物学的メカニズムの検討 (2012)
  • Author(s): 犬伏俊博、宮内睦美、敖敏、石角篤、牧野武利、高田隆
  • Organizer: 第5回日本ラクトフェリン学会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-27
• [Presentation] ラクトフェリンはマクロファージのTNE-a産生を抑制する (2012)
  • Author(s): 西山今日子、犬伏俊博、宮内睦美、敖敏、石角篤、牧野武利、高田隆
  • Organizer: 第5回日本ラクトフェリン学会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-27
• [Presentation] ラクトフェリンによる骨吸収抑制作用とその分子生物学的メカニズムの検討硬組織再生生物学会 (2012)
  • Author(s): 犬伏俊博、宮内睦美、敖敏、石角篤、牧野武利、高田隆
  • Organizer: 第5回日本ラクトフェリン学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-08-25