2013 Fiscal Year Final Research Report
The search for the master regulatory genes for tooth development using iPS cell derived teratomas
| Project/Area Number |
24659819
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Naoki 岩手医科大学, 歯学部, 准教授 (20190100)
OTSU Keishi 岩手医科大学, 歯学部, 助教 (60509066)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | iPS細胞 / 奇形種 / 歯 / 発生 / 再生医学 |
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| Research Abstract |
iPS cells-derived teratomas do not contain tooth germ. Therefore, we hypothesized that we could induce tooth development from the stem cells in the teratomas by the introduction of exogenous tooth morphogenesis-related genes. During teratoma formation, iPS cells differentiated into epithelial cells and formed various types of epithelial tissue. Our results also indicate that these epithelia included epithelial stem/progenitor cell marker positive cells. Further, we succeeded in the introduction of tooth germ epithelium specific gene, Sp-6, into the teratomas, and which induced epithelial differentiation. These results will develop innovations for the study of tooth regeneration.
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