2013 Fiscal Year Final Research Report
Dvelopment of novel adjuvant which constructed by DNA origami technology
| Project/Area Number |
24659836
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Niigata University |
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Principal Investigator |
TERAO Yutaka 新潟大学, 医歯学系, 教授 (50397717)
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Shigetada 大阪大学, 大学院歯学研究科, 教授 (50273694)
NAKATA Masanobu 大阪大学, 大学院歯学研究科, 准教授 (90444497)
SUMITOMO Tomoko 大阪大学, 大学院歯学研究科, 助教 (50423421)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | アジュバント |
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| Research Abstract |
Recently, pathogenic microorganisms acquired drug-resistance, and they are spreading throughout the world. Antibiotics were presented as "magic bullets" against infectious diseases in the 20th century, however, the heavy usage of antibiotics has led to the appearance of drug-resistant microorganisms. Thus, it has become difficult to treat most infantile diarrhea with basic antibiotics in the developing countries. Now, it is important to develop and improve the enteric and general immune activators on the basis of fundamental investigations. In this proposal, we focus on a CpG motif among the DNA sequence. The CpG motif is observed at high frequency in the genomes of pathogenic microorganisms. It works as an immune activator to boost production of antigen-specific immune responses. In this study, we constructed "DNA origami" immune activators being fixed the GpG motifs to the outside using M13 DNA, aiming to obtain the immunopotentiating effect.
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[Journal Article] Penetration kinetics of four mouthrinses into Streptococcus mutans biofilms analyzed by direct time-lapse visualization2014
Author(s)
Wakamatsu, R., Takenaka, S., Ohsumi, T., Terao, Y., Ohshima, H., and Okiji, T
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Journal Title
Clin. Oral Invest
Volume: 8
Pages: 625-34
Peer Reviewed
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[Journal Article] STO feeder cells are useful for propagation of primarily cultured human deciduous dental pulp cells in view of elimination of contaminated bacteria and promotion of cellular outgrowth2013
Author(s)
Murakami, T., Saitoh, I., Inada, E., Kurosawa, M., Iwase, Y., Noguchi, H., Terao, Y., Yamasaki, Y., Hayasaki, H., and Sato, M
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Journal Title
Cell Medicine
Volume: 6
Pages: 75-81
Peer Reviewed
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[Journal Article] Streptococcus pneumoniae invades erythrocytes and utilizes them to evade human innate immunity2013
Author(s)
Yamaguchi, M., Terao, Y., Mori-Yamaguchi, M., Domon, H., Sakaue, Y., Yagi, T., Nishino, K., Yamaguchi, A., Nizet, V., and Kawabata, S
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Journal Title
PLoS one
Volume: 8
Pages: 77282
Peer Reviewed
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[Journal Article] Cysteine proteinase from Streptococcus pyogenes enables to evade innate immunity via degradation of complement factors2013
Author(s)
Honda-Ogawa, M., Ogawa, T., Terao, Y., Sumitomo, T., Nakata, M., Ikebe, K., Maeda, Y., and Kawabata, S
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Journal Title
J. Biol. Chem
Volume: 288
Pages: 15854-15864
Peer Reviewed
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[Journal Article] α-enolase of Streptococcus pneumoniae induces formation of neutrophil extracellular traps2012
Author(s)
Mori, Y., Yamaguchi, M., Terao, Y., Hamada, S., Ooshima, T., and Kawabata, S
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 10472-10481
Peer Reviewed
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[Journal Article] Long-term survival of salivary streptococci on dental devices made of EVA2012
Author(s)
Ogawa, T., Yamasaki, S., Honda, M., Terao, Y., Kawabata, S., and Maeda, Y
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Journal Title
Int J. Oral Science
Volume: 4
Pages: 14-8
Peer Reviewed
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[Journal Article] Involvement of T6 pili in biofilm formation by serotype M6 Streptococcus pyogenes2012
Author(s)
Kimura, K. R., Nakata, M., Sumitomo, T., Kreikemeyer, B., Podbielski, A., Terao, Y., and Kawabata, S
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Journal Title
J. Bacteriol
Volume: 194
Pages: 804-812
Peer Reviewed
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[Journal Article] Neonatal pleural empyema caused by emm type 6 group A streptococcus
Author(s)
Nohara, F., Nagaya, K., Asai, H., Tsuchida, E., Okamoto, T., Hayashi, T., Sakata, H., Terao, Y., and Azuma, H
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Journal Title
Pediatirics International
Volume: (in press)
Peer Reviewed
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