2013 Fiscal Year Final Research Report
Mechanistic analysis of periodontal bacteria attached on cardiovascular tissues by live-imaging
| Project/Area Number |
24659840
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TSUKUBA Takayuki 長崎大学, 医歯(薬)学総合研究科, 教授 (30264055)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Kuniaki 長崎大学, 大学院医歯薬学総合研究科, 准教授 (10311846)
SAKAI Eiko 長崎大学, 大学院医歯薬学総合研究科, 助教 (10176612)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ライブイメージング / 歯周病細菌 |
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| Research Abstract |
We analyzed a fate of Porphyromonas gingivalis, a periodontal pathogen, with human aortic endothelial cells by live-imaging. We detected the bacteria within the cells for 6-8 hs, but failed to find them for 18-24 hs, indicating that the bacteria is probably transported to the lysosomes.
Futhermore, we performed live-imaging of P. gingivalis using mice. We found that the bacteria attached on blood vessels of bifurcation area and thin vessels. In ApoE-deficient mice, which is a model for arterial sclerosis, the attachment rate of the bacteria was increased. These results indicate that cholesterol in the blood probably interacts with P. gingivalis during endocytosis.
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[Journal Article] Repression of cathepsin E expression increases the risk of mammary carcinogenesis and links to poor prognosis in breast cancer2014
Author(s)
Kawakubo T, Yasukochi A, Toyama T, Takahashi S, Okamoto K, Tsukuba T, Nakamura S, Ozaki Y, Nishigaki K, Yamashita H, Yamamoto K
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Journal Title
Carcinogenesis
Volume: 35(3)
Pages: 714-126
Peer Reviewed
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