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2013 Fiscal Year Final Research Report

Development of biopolymer compound to elucidate the effect of glucan on innate immune system

Research Project

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Project/Area Number 24659841
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionKyushu Dental College

Principal Investigator

NISHIHARA Tatsuji  九州歯科大学, 歯学部, 教授 (80192251)

Co-Investigator(Kenkyū-buntansha) ARIYOSHI Wataru  九州歯科大学, 歯学部, 准教授 (40405551)
Co-Investigator(Renkei-kenkyūsha) OKINAGA Toshinori  九州歯科大学, 歯学部, 助教 (60582773)
Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsマクロファージ / β1-3グルカン / dectin-1 / インフラマソーム
Research Abstract

Several immune system cell-surface receptors are reported to be associated with osteoclastogenesis. Dectin-1, a lectin receptor for beta-glucan, is found predominantly on cells of the myeloid lineage. In the present study, we examined the effect of dectin-1 agonist, curdlan, on osteoclastogenesis. In dectin-1 over-expressing RAW 264.7 cells (d-RAW), curdlan suppressed receptor activator of nuclear factor-kappaB (NF-kappaB) ligand (RANKL)-induced osteoclast differentiation, bone resorption and actin-ring formation. Furthermore, curdlan inhibited RANKL-induced nuclear factor of activated T-cell cytoplasmic 1 (NFATc1) expression.
In conclusion, our results demonstrate that curdlan potentially inhibits osteoclast differentiation, especially NFATc1 expression. This suggests activation of curdlan-dectin-1 interaction critically regulates the genes required for osteoclastogenesis.

  • Research Products

    (55 results)

All 2014 2013 2012 Other

All Journal Article (14 results) (of which Peer Reviewed: 11 results) Presentation (40 results) Remarks (1 results)

  • [Journal Article] High Molecular Weights Hyaluronic Acid Regulates Osteoclast Formation by Inhibiting Receptor Activator of NF-kappaB Ligand through Rho Kinase2014

    • Author(s)
      Ariyoshi W, Okinaga T, Knudson CB, Knudson W, Nishihara T
    • Journal Title

      Osteoarthritis cartilage

      Volume: 22(1) Pages: 111-20

    • DOI

      10.1016/j.joca.2013.10.013

    • Peer Reviewed
  • [Journal Article] Factors associated with dry mouth in dependent Japanese elderly2014

    • Author(s)
      Kakudate N, Muramatsu T, Endoh M, Satomura K, Koseki T, Sato Y, Ito K, Ogasawara T, Nakamura S, Kishimoto E, Kashiwazaki H Yamashita Y, Uchiyama K, Nishihara T, Kiyohara Y, Kakinoki Y
    • Journal Title

      Gerodontology

      Volume: 31(1) Pages: 11-18

    • DOI

      10.1111/j.1741-2358.2012.00685.x

    • Peer Reviewed
  • [Journal Article] Develop- ment of a Membrane-based Micro- wave-mediated Electrochemical ELISA Method for TNF-αDetection in Patients with Periodontitis2013

    • Author(s)
      Diala I, Sato S, Usui M, Nakashima K, Nishihara T, Takenaka S
    • Journal Title

      Anal Sci

      Volume: 29(9) Pages: 917-930

    • URL

      https://www.jstage.jst.go.jp/article/analsci/29/9/29_927/_pdf

    • Peer Reviewed
  • [Journal Article] Oral cancer diagnosis via a ferrocenylnaphthalene diimide- based electrochemical telomerase assay2013

    • Author(s)
      Mori K, Sato S, Kodama M, Habu M, Takahashi O, Nishihara T, Tominaga K, Takenaka S
    • Journal Title

      Clin Chem

      Volume: 59(1) Pages: 289-295

    • DOI

      10.1373/clinchem.2012.191569

    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide of Aggregatibacter actinomycetemcomi- tans upregulates inflammatory cytokines, PGE2 synthesis, and osteoclast formation in IL-1Ra deficient mice2013

    • Author(s)
      Mizutani H, Ishihara Y, Izawa A, Fujihara Y, Kobayashi S, Gotou H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Kamei H, Kikuchi T, Yamamoto G, Mitani A, Nishihara T, Noguchi T
    • Journal Title

      J Periodont Res

      Volume: 48(6) Pages: 748-756

    • DOI

      10.1111/jre.12065

    • Peer Reviewed
  • [Journal Article] Mechanisms of G1 cell cycle arrest and apoptosis in myeloma cells induced by hybrid-compound histone deacetylase inhibitor2013

    • Author(s)
      Fujii S, Okinaga T, Ariyoshi W, Takahashi O, Iwanaga K, Nishino N, Tominaga K, Nishihara T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 434(3) Pages: 413-420

    • DOI

      10.1016/j.bbrc.2013.03.043

    • Peer Reviewed
  • [Journal Article] Mechanisms involved in suppression of ADAMTS4 expression in synoviocytes by high molecular weight hyaluronic acid2013

    • Author(s)
      Kataoka Y, Ariyoshi W, Okinaga T, Kaneuji T, Mitsugi S, Takahashi T, Nishihara T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 432(4) Pages: 580-585

    • DOI

      10.1016/j.bbrc.2013.02.043

    • Peer Reviewed
  • [Journal Article] A actinomycetemcomitans LPS enhances form cellformation induced by LDL2013

    • Author(s)
      Morishita M, Ariyoshi W, Okinaga T, Usui M, Nakashima K, Nishihara T
    • Journal Title

      J Dent Res

      Volume: 92(3) Pages: 241-246

    • DOI

      10.1177/0022034512473309

    • Peer Reviewed
  • [Journal Article] Essential role of JAK/STAT pathway in the induction of cell cycle arrest in macrophages infected with periodontopathic bacterium Aggregatibacter actinomycetemcomitans2013

    • Author(s)
      Okinaga T, Ariyoshi W, Akifusa S, Nishihara T
    • Journal Title

      Medical Microbiol Immunol

      Volume: 202(2) Pages: 167-174

    • DOI

      10.1007/s00430-012-0282-x

    • Peer Reviewed
  • [Journal Article] Mechanisms involved in inhibition of chondrogenesis by activin-A2013

    • Author(s)
      Mitsugi S, Ariyoshi W, Okinaga T, Kaneuji T, Kataoka Y, Takahashi T, Nishihara T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 420(2) Pages: 380-384

    • DOI

      10.1016/j.bbrc.2012.03.003

    • Peer Reviewed
  • [Journal Article] 歯周炎におけるマクロファージの機能の多様性2012

    • Author(s)
      有吉渉,西原達次
    • Journal Title

      第3回炎症性骨吸収に対するマクロファージの関与日本歯科評論

      Volume: 第842号 Pages: 149-152

  • [Journal Article] 歯周炎におけるマクロファージの機能の多様性2012

    • Author(s)
      沖永敏則,西原達次
    • Journal Title

      第2回マクロファージにおける炎症応答でのインフラマソームの役割日本歯科評論

      Volume: 第841号 Pages: 153-155

  • [Journal Article] 歯周炎におけるマクロファージの機能の多様性第1回マクロファージの機能に対するアディポネクチンの役割と歯周疾患との関連2012

    • Author(s)
      秋房住郎,西原達次
    • Journal Title

      日本歯科評論

      Volume: 第840号 Pages: 155-157

  • [Journal Article] Quantitative discrimination of Aggregatibacter actinomycetemcomitans highly leukotoxic JP2 clone from non-JP2 clones in diagnosis of aggressive periodontitis2012

    • Author(s)
      Yoshida A, Ennibi OK, Miyazaki H, Hoshino T, Hayashida H, Nishihara T, Awano S, Ansai T
    • Journal Title

      BMC Infect Dis

      Volume: 12 Pages: 253

    • DOI

      10.1186/1471-2334-12-253

    • Peer Reviewed
  • [Presentation] Dectin-1 activation inhibits an inflammatory response to bacterial infection2014

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Year and Date
      20140326-28
  • [Presentation] 新規アディポカインapelinによる炎症反応に対する影響2013

    • Author(s)
      小原成将, 秋房住郎, 臼井通彦, 笠井宏記, 有吉渉, 沖永敏則, 西原達次
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      20130920-22
  • [Presentation] Curdlan-dectin-1を介した新たな破骨細胞分化の制御機構2013

    • Author(s)
      山﨑徹,有吉渉,沖永敏則,細川隆司,西原達次
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      20130920-22
  • [Presentation] 象牙芽細胞様細胞に対する多血小板血漿の作用2013

    • Author(s)
      廉炅勲,鷲尾絢子,有吉渉,北村知昭,西原達次
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      20130920-22
  • [Presentation] アメロブラスチンは口腔上皮細胞の細胞増殖を抑制する2013

    • Author(s)
      西藤法子, 有吉渉, 沖永敏則, 鷲尾絢子, 北村知昭, 西原達次
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      20130920-22
  • [Presentation] 歯周病細菌感染マクロファージにおけるインフラマソーム活性2013

    • Author(s)
      沖永敏則,有吉渉,西原達次
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      20130920-22
  • [Presentation] Inflammasome activation via periodontopathic bacterial infection in macrophage2013

    • Author(s)
      Okinaga T, Ariyoshi W, Usui M, Nishihara T
    • Organizer
      2^<nd> meeting of IADR-Asia Pathific Region
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      20130821-23
  • [Presentation] 新規アディポカインapelinはLPSの誘導する炎症反応を抑制する2013

    • Author(s)
      小原成将, 秋房住郎, 臼井通彦, 笠井宏記, 中島啓介, 西原達次
    • Organizer
      第56回春季日本歯周病学会学術大会
    • Place of Presentation
      船堀
    • Year and Date
      20130531-0601
  • [Presentation] High molecular weight hyaluronan down-regulates the expression of ADAMTS4 via p38 MAPK signaling pathway in human synoviacytes2013

    • Author(s)
      Kataoka Y, Ariyoshi W, Okinaga T, Nishihara T
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20130528-0601
  • [Presentation] Curdlan regulates osteoclastic differenti- ation and function2013

    • Author(s)
      Yamazaki T, Ariyoshi W, Okinaga T, Hosokawa R, Nishihara T
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20130528-0601
  • [Presentation] Inhibitory effect of hyaluronic acid on osteoclastogenesis via Rho kinase2013

    • Author(s)
      Ariyoshi W, Okinaga T, Nishihara T
    • Organizer
      2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20130528-0601
  • [Presentation] Inhibitory Effect of Ameloblastin on Epithelial Cells2013

    • Author(s)
      Saito N, Washio A, Nishihara T, Kitamura C
    • Organizer
      IFEA
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20130523-26
  • [Presentation] Effects of Newly Developed Bioglass Cement on Cell Morphology and Viability2013

    • Author(s)
      Nakagawa A, Washio A, Hirata-Tsuchia S, Terashita M, Nishihara T, Kitamura C
    • Organizer
      IFEA
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20130523-26
  • [Presentation] CurdlanはDectin-1を介して破骨細胞形成を抑制する2013

    • Author(s)
      山﨑徹,有吉渉,正木千尋,中本哲自,西原達次,細川隆司
    • Organizer
      第122回日本補綴歯科学会学術大会
    • Place of Presentation
      福岡
    • Year and Date
      20130518-19
  • [Presentation] PC12細胞の神経細胞分化に対する各種セメントの影響2013

    • Author(s)
      中川愛加,沖永敏則,鷲尾絢子,寺下正道,北村知昭,西原達次
    • Organizer
      第74回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20130518-19
  • [Presentation] アメロブラスチン発現による上皮細胞増殖への影響について2013

    • Author(s)
      西藤法子, 蔵満幸子, 有吉渉, 沖永敏則, 北村知昭, 山口和憲, 西原達次
    • Organizer
      第74回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20130518-19
  • [Presentation] 新規アディポカインapelinの免疫機能に対する影響について2013

    • Author(s)
      小原成将, 秋房住郎, 臼井通彦, 笠井宏記, 有吉渉, 沖永敏則, 西原達次, 中島啓介
    • Organizer
      第74回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20130518-19
  • [Presentation] 象牙芽細胞様細胞の抗炎症能と分化能に与える多血小板血漿の影響2013

    • Author(s)
      廉炅勲,鷲尾絢子,有吉渉,北村知昭,西原達次
    • Organizer
      第74回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20130518-19
  • [Presentation] The effect of HDAC inhibitor on mouse myeloma cells2013

    • Author(s)
      Fujii S, Okinaga T, Ariyoshi W, Takahashi O, Iwanaga K, Habu M, Tominaga K, Nishihara T
    • Organizer
      IADR
    • Place of Presentation
      Seattle, USA
    • Year and Date
      20130320-23
  • [Presentation] High-molecular-weight hyaluronic acid down-regulates the expression of ADAMTS4 in synoviocytes2013

    • Author(s)
      Kataoka Y, Ariyoshi W, Okinaga T, Kaneuji T, Takahashi T, Nishihara T
    • Organizer
      IADR
    • Place of Presentation
      Seattle, USA
    • Year and Date
      20130320-23
  • [Presentation] Role of ameloblastin on epithelial cells prolifetration2013

    • Author(s)
      Saito N, Kuramitsu S, Washio A, Ariyoshi W, Kitamura C, Nishihara T
    • Organizer
      IADR
    • Place of Presentation
      Seattle, USA
    • Year and Date
      20130320-23
  • [Presentation] Effect of Dectin-1 signaling on osteoclasto- genesis2013

    • Author(s)
      Yamasaki T, Ariyoshi W, Okinaga T, Hosokawa R, Nishihara T
    • Organizer
      IADR
    • Place of Presentation
      Seattle, USA
    • Year and Date
      20130320-23
  • [Presentation] Streptococcus sanguinisはマクロファージの泡沫化と細胞死を誘導する2013

    • Author(s)
      岡橋暢夫,沖永敏則,桜井敦朗,寺尾豊,中田匡宣,川端重忠,西原達次
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張
    • Year and Date
      20130318-20
  • [Presentation] 歯周病細菌の生育を抑制・阻害する緑膿菌分泌物の作用機構の追及2013

    • Author(s)
      常岡祐介,前田憲成,沖永敏則,西原達次
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張
    • Year and Date
      20130318-20
  • [Presentation] 歯周病原性細菌マクロファージのピロトーシス誘導におけるインフラマソームの役割2013

    • Author(s)
      沖永敏則, 有吉渉, 西原達次
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張
    • Year and Date
      20130318-20
  • [Presentation] Periodontal diseases in elderly patients2013

    • Author(s)
      Nishihara T
    • Organizer
      The Scientific Meeting of TADOH
    • Place of Presentation
      Kaohsiung, Taiwan
    • Year and Date
      2013-09-16
  • [Presentation] High molecular weights hyaluronic acid regulates osteoclastogenesis2013

    • Author(s)
      Ariyoshi W, Okinaga T, Knudson CB, Knudson W, Nishihara T
    • Organizer
      Hyaluronan 2013
    • Place of Presentation
      Oklahoma, USA
    • Year and Date
      2013-06-07
  • [Presentation] New detection system for the adhesion of oral bacteria on macrophages in vitro2013

    • Author(s)
      Morishita M, Okinaga T, Ariyoshi W, Nakashima K, Nishihara T
    • Organizer
      International Symposium on Oral Education and Research in Kitakyushu
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2013-01-26
  • [Presentation] The involvement of inflammasome in mouse macrophage infected with Aggregatibacter actinomycetem- comitans2013

    • Author(s)
      Okinaga T, Ariyoshi W, Nishihara T
    • Organizer
      International Symposium on Oral Education and Research in Kitakyushu
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2013-01-26
  • [Presentation] 破骨細胞支持能に対する高分子ヒアルロン酸の抑制効果について2012

    • Author(s)
      有吉渉,沖永敏則,西原達次
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      群山
    • Year and Date
      20120914-16
  • [Presentation] 歯面初期定着菌群との共培養におけるStreptococcus mutans抗酸化タンパク質の機能解析2012

    • Author(s)
      安永愛,吉田明弘,西原達次,安細敏弘
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      群山
    • Year and Date
      20120914-16
  • [Presentation] 歯周病原性細菌はマクロファージにおけるpyroptosisを誘導する2012

    • Author(s)
      沖永敏則,有吉渉,西原達次
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      群山
    • Year and Date
      20120914-16
  • [Presentation] 破骨細胞文化におけるDectin-1の作用について2012

    • Author(s)
      山崎徹,有吉渉,沖永敏則,細川隆司,西原達次
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      群山
    • Year and Date
      20120914-16
  • [Presentation] マクロファージ及び滑膜細胞におけるプロテアーゼ産生に及ぼすヒアルロン酸の影響2012

    • Author(s)
      片岡良浩,有吉渉,西原達次
    • Organizer
      第33回日本炎症・再生医学会
    • Place of Presentation
      福岡
    • Year and Date
      20120705-06
  • [Presentation] マクロファージ貪食機能ににおけるデクチンの役割について2012

    • Author(s)
      井上真紀,藤井誠子,片岡良浩,守下昌輝,沖永敏則,有吉渉,西原達次
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20120519-20
  • [Presentation] 歯・歯周組織疾患と動脈硬化の関連について2012

    • Author(s)
      中島啓介,北村知昭,西原達次
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20120519-20
  • [Presentation] 歯科医療産業への展開を目指した根管および歯周組織の微細構造観察用高解像度デバイスの開発2012

    • Author(s)
      北村知昭,吉居慎二,寺下正道,西原達次
    • Organizer
      第72回九州歯科学会総会
    • Place of Presentation
      北九州
    • Year and Date
      20120519-20
  • [Presentation] 口腔内における難治性感染症,耐性菌感染症の治療2012

    • Author(s)
      西原達次, 金子明寛
    • Organizer
      第60回日本化学療法学会学術集会シンポジウム
    • Place of Presentation
      長﨑
    • Year and Date
      20120426-27
  • [Presentation] Physiochemical properties of newly developed bioactive glass cement and its effect on various cell responses2012

    • Author(s)
      Washio A, Nakagawa A, Nishihara T,Kitamura C
    • Organizer
      The fifth Japan-Korea Joint Symposium on Bio- microsensing Technology
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2012-10-26
  • [Presentation] A simple approach to test periodontal disease frpm saliva using fourier transporform infrared spectroscopy2012

    • Author(s)
      Fujii S, Fukuda K, Okinaga T, Ariyoshi W, Nakashima K, Nishihara T, Sarai A, Sato S, Takenaka S
    • Organizer
      The fifth Japan-Korea Joint Symposium on Bio-microsensing Technology
    • Place of Presentation
      Kitakyushu, Japan
    • Year and Date
      2012-10-26
  • [Remarks]

    • URL

      http://www.kyu-dent.ac.jp/research/lecture/infection_molecule

URL: 

Published: 2015-06-25  

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