2013 Fiscal Year Final Research Report
Assembly of recellularized-dental pulp models using stem cells and decellularized dental pulp matrix
| Project/Area Number |
24659849
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Aichi Gakuin University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OZEKI Nobuaki 愛知学院大学, 歯学部, 講師 (70469005)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 脱細胞化 / 再細胞化 / ES細胞 / iPS細胞 / 骨格筋幹細胞 |
|---|
| Research Abstract |
The decellularized dental pulp-mimicking matrices (ddpm) was extracted and purified by 0.1% sodium dodecyl sulfate (SDS). After the cells were seeded on ddpm for 7 days, cells exhibited neural crest cell markers (FoxD3, Sox10), neural progenitor cell markers (Notch-1, Nestin), odontoblast differentiation markers (Dspp, Dmp-1, Enamelysin) and vascular endothelial cell markers (Flk-1, VE-cadherin) by reverse transcription (RT)-polymerase chain reaction (PCR).
we conclude that the parental stem cells retained a specification that following ddpm treatment shifted to platform differentiated to dental pulp-like specification.
Possible alternative stem cells from other tissues including skeletal muscle could potentially be adapted for tooth organ regeneration using ddpm and these new approaches for teeth regeneration needs to be explored.
|
