2012 Fiscal Year Final Research Report
he clarification of the mechanism of postoperative bone resorption using simulated microgravity machine.
| Project/Area Number |
24659865
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMOTO Tetsuji 九州歯科大学, 歯学部, 准教授 (30514989)
MASAKI Chihiro 九州歯科大学, 歯学部, 助教 (60397940)
KONDO Yusuke 九州歯科大学, 歯学部, 助教 (00611287)
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| Project Period (FY) |
2012
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| Keywords | 微小重力 / メカノバイオロジー |
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| Research Abstract |
In this study, we tried to clarify the mechanism of unloading-induced bone loss in some sort by mechano-biological method using simulated microgravity machine. Murine gingival epithelial cell line, GE1, were cultured in both groups ,control (1G) group and micro gravity (CL) group. Cells were cultured continuously for 10 days and sampling were done on days 0, 3, 5, 7, 10, each taking 3 samples from each group. In result, CL group demonstrated inhibition of cell proliferation to half of that of 1G group. Also, morphological differences were suggested since cells in the 1G group were observed flat in uniform surface while cells in the CL group showed distinct cell boundary.
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