Direct reprogramming to osteocytes by modulating insulator function

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659870
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IKEDA Masa-Aki 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20193211)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 分化転換 / リプログラミング / インスレーター / 骨細胞 / ARID3A / 繊維芽細胞 / 間葉系幹細胞 / 再生医療

Outline of Final Research Achievements

In this study, we tried to inhibit insulator functions by siRNA-mediated ARID3A silencing and thereby facilitate reprogramming. In order to convert human fibroblasts into osteocytes, we tested the effects of siRNA-mediated ARID3Asilencing and various combinations of small chemical compounds and growth factors on epigenetic reprogramming of fibroblasts. We found a combination of small chemical compounds and growth factors that directly converted fibroblasts into osteocytes and adipocytes without using siRNA-mediated ARID3A silencing.

Free Research Field

医歯薬学