Challenges to tissue regeneration using adipose-derived spheroids.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Iwate Medical University (2014); Tokyo Medical and Dental University (2012-2013)
• Principal Investigator: NAKATA Hidemi 岩手医科大学, 歯学部, 非常勤講師 (30451967)
• Co-Investigator(Kenkyū-buntansha): KURODA Shinji 東京医科歯科大学, 大学院医歯学総合研究科口腔機能再構築学講座インプラント・口腔再生医学分野, 助教 (50323689) ; KONDO Hisatomo 岩手医科大学, 歯学部補綴・インプラント学講座, 教授 (70343150)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脂肪由来幹細胞(ASC) / スフェロイド / 構造解析 / 共培養 / 単球(monocytes)

Outline of Final Research Achievements

Among potential cell-based therapies, adipose-derived stem cells (ASCs) have thought as a promising source of stem cells for tissue regeneration because it contains approximately 1000-fold more stem cells than the bone marrow.
In the present study, we analyzed the structures and expression of marker genes for multipotency, including Oct3/4, Nanog, and Sox2 of murine-derived ASC macrospheroids by in situ hybridization. The microstructures of the ASC macrospheroids were observed by transmission electron microscopy, and cell proliferation in the spheroids was analyzed by enzyme-linked immunoassay for bromodeoxyuridine as well as the growth and osteogenic potential of these macrospheroids in three-dimensional environment and following induction of calcification by co-culture with monocytes.
These results suggested that ASC macrospheroids presented high capacity for dynamic three-dimensional growth, and it might be promoted its osteogenic potential by monocytes.(Preparing for publication)

Free Research Field

再生医療