New development of clinical dental treatment that uses ceramics with cell compatibility and fish collagen

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659879
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: IKEDA Kahori 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (20578330)
• Co-Investigator(Kenkyū-buntansha): SUGIMOTO Kouji 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (40646113)
• Co-Investigator(Renkei-kenkyūsha): YOSHIZAWA Yuu 長崎大学, 病院(歯学系), 医員 (60746931)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: セラミックス / コラーゲン / 細胞移植 / 象牙質 / 再生医療

Outline of Final Research Achievements

Marine collagen derived fromfish scales, skin, and bone has been widely investigated for application as a scaffold and carrier due to its bioactive properties, including excellent biocompatibility, low antigenicity, and high biodegradability and cell growth potential.
This study was conducted to confirmthe safety of fish (tilapia) atelocollagen for use in clinical application.We performed in vitro and in vivo biological studies of medical materials to investigate the safety of fish collagen.The extract of fish collagen gel was examined to clarify its sterility. All present sterility tests concerning bacteria and viruses (including endotoxin) yielded negative results, and all evaluations of cell toxicity, sensitization, chromosomal aberrations, intracutaneous reactions, acute systemic toxicity, pyrogenic reactions, and hemolysis were negative.The present study demonstrated that atelocollagen prepared from tilapia is a promising biomaterial for use as a scaffold in regenerative medicine.

Free Research Field

生体工学