2013 Fiscal Year Final Research Report
Genomic analysis of the 11q13.3 amplicon navigation microdissection in oral cancer
| Project/Area Number |
24659889
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OMURA KEN 東京医科歯科大学, 医歯学総合研究科, 教授 (10334434)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Keiichi 東京医科歯科大学, 硬組織疾患ゲノムセンター, 特任講師 (10396971)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 口腔がん / ゲノム / マイクロアレイ / 11q13.3 / マイクロダイセクション |
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| Research Abstract |
The aim is to develop the methods that detect the genomic structure abnormality in oral cancer cell with high-level genomic abnormality. We were confirming the satisfactory data of CGH microarray analysis using extracted 100ng input DNA from a formalin fixation paraffin embedded (FFPE) tissue. Moreover, we were confirming the satisfactory data of CGH microarray analysis using extracted DNA from a sample mounted in slide-glass which coverslip on the slide were removed by soaking the slide in xylene after immunohistostaining. To detect the genomic abnormality using smaller quantity of input DNA, we performed the targeted next-generation sequencing. We detected the genomic abnormality in oral cancer from 15ng input DNA.
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[Presentation] 口腔扁平上皮癌患者のゲノム構造異常解析による治療抵抗性予測2013
Author(s)
森田圭一,松川祥,原田浩之,中島雄介,島本裕彰,富岡寛文,田中香衣,林深,小崎健一,稲澤譲治,小村健
Organizer
第37回日本頭頸部癌学会
Place of Presentation
東京
Year and Date
20130613-14
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