Establishment of salivary gland regeneration method using salivary gland tissue-originated spheroid (STOS)

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659899
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: SUGIURA Tsuyoshi 九州大学, 歯学研究科(研究院), 准教授 (40322292)
• Co-Investigator(Kenkyū-buntansha): IKARI Tatsuya 九州大学, 大学病院, 助教 (70380467)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 幹細胞 / 唾液腺再生

Research Abstract

We demonstrated the regenerative capability of dissociated E13.5 embryonic submandibular gland (SMG) cells in vitro in collagen type I gels. The resulting structures were similar to native salivary glands. Large cyst-like structures, probably due to swollen ducts, began to appear on day 7, and they enlarged and increased in number by day 14. Through the addition of fibronectin and an anti- integrin antibody into the regenerate salivary glands, cyst formation and enlargement were clearly inhibited, and the expression and localization of AQP5 was concentrated on the lumen side with cell polarity similar to native salivary glands. In contrast, anti- integrin antibodies induced cyst formation and enlargement. Together, these data indicate that dissociated SMGs can regenerate salivary gland structure, and specific interactions between integrins and extracellular matrices are essential for regenerate salivary gland differentiation and development.