Biological study on DMP1 based on molecular evolutionary medicine

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659918
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: SHINTANI Seikou 東京歯科大学, 歯学部, 教授 (90273698)
• Co-Investigator(Kenkyū-buntansha): SAKURAI Atsuo 東京歯科大学, 歯学部, 講師 (90431759)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: DMP1 / 分子進化 / 進化医学

Research Abstract

The purpose of this study was to identify and characterize an ortholog of the DMP1 gene in an amphibian (Xenopus laevis; X. laevis) to clarify molecular evolutionary alterations in DMP1. Furthermore, we analyzed the mRNA expression of this gene to elucidate its functional change in bone and developing tooth germ in comparison with amniote DMP1s. They shared several unique features specific to DMP1 and have similar properties. Expression of X. laevis DMP1 mRNA was predominant in osteocytes and odontoblasts, but only transiently observed in ameloblasts, as in amniotes. These results suggest that DMP1 has conserved several functions during tetrapod evolution. This indicates that continuity of biochemical properties has been more important in maintaining DMP1 functionality than that of the sequence of amino acid residues, which has undergone change over the course of molecular evolution.

Research Products

• [Journal Article] 知らずに日常臨床で遭遇している (2014)
  • Author(s): 新谷誠康
  • Journal Title: "MIH"を知る,ザ・クインテッセンス33 Volume: (1) Pages: 150-155
• [Journal Article] Shintani S, Association between feeding methods and sucking habitsa cross-sectional study of infants in their first 18 months of life (2013)
  • Author(s): Yonezu T, Arano-Kojima T, Kumazawa K
  • Journal Title: Bull Tokyo Dent Coll Volume: 54(4) Pages: 215-21
  • Peer Reviewed
• [Journal Article] Longitudinal investigation of relationship between developmental changes in sagittal occlusion and caries in lower first permanent molars (2013)
  • Author(s): Yonezu T, Kojima T, Kumazawa K, Shintani S
  • Journal Title: Bull Tokyo Dent Coll Volume: 54(4) Pages: 209-13
  • Peer Reviewed
• [Journal Article] Identification, characterization, and expression of dentin matrix protein 1 gene in Xenopus laevis (2013)
  • Author(s): Yonekura T, Homma H, Sakurai A, Moriguchi M, Miake Y, Toyosawa S, Shintani S
  • Journal Title: J. Exp. Zool Volume: 320(8) Pages: 525-537
  • Peer Reviewed
• [Journal Article] Effect of combination of fused teeth on eruption of permanent successors (2013)
  • Author(s): Tsujino K, Yonezu T, Shintani S
  • Journal Title: Pediatr Dent Volume: 35(2) Pages: 64-7
  • Peer Reviewed
• [Journal Article] 乳歯が生えてきた!ばっちりわかるここからのケア&指導知っておきたい!この時期の歯の異常・萌出に関する異常・構造の異常 (2013)
  • Author(s): 今井裕樹、新谷誠康
  • Journal Title: 歯科衛生士 Volume: 37(4) Pages: 58-59
• [Journal Article] 乳歯の癒合歯が後継永久歯に与える影響 (2013)
  • Author(s): 新谷誠康
  • Journal Title: 日本歯科医師会雑誌 Volume: 65(12)(カラーグラビアp.1) Pages: 6-14
• [Journal Article] 楽木正実,自閉症者に対し糸身体抑制法を選択するための基準 (2012)
  • Author(s): 大西智之,久喜富美子,新谷誠康,今井裕樹
  • Journal Title: 障歯誌 Volume: 33(4) Pages: 632-639
  • Peer Reviewed
• [Journal Article] Organization of Mhc class II A and B genes in the tilapiine fish Oreochromis niloticus and evidence for their co-evolution (2012)
  • Author(s): Sato A, Dongak R, Hao L, Shintani S, Sato T
  • Journal Title: Immunogenetics Volume: 64(9) Pages: 679-690
  • Peer Reviewed
• [Journal Article] Ultrastructural immunolocalization of dentin matrix protein 1 on Sharpey's fibers in monkey tooth cementum (2012)
  • Author(s): Sawada T, Ishikawa T, Shintani S, Yanagisawa T
  • Journal Title: Biotech Histochem Volume: 87(5) Pages: 360-365
  • Peer Reviewed
• [Journal Article] Effect of single-dose amoxicillin on rat incisor odontogenesis: a morphological, study (2012)
  • Author(s): Kumazawa K, Sawada T, Yanagisawa T, Shintani S
  • Journal Title: Clin Oral Investig Volume: 16(3) Pages: 835-842
  • Peer Reviewed
• [Journal Article] Missense mutation of EDA1 gene in Japanese family with X-linked anhidrotic ectodermal dysplasia (2012)
  • Author(s): Sekiguchi H, Senzui S, Yamashita H, Shintani S, Sawada T, Yanagisawa T
  • Journal Title: Ped. Dent. J Volume: 22 (2) Pages: 188-192
  • Peer Reviewed
• [Journal Article] Marginal periodontitis with alveolar bone loss in lower deciduous incisor due to drinking straw-like plastic tube (2012)
  • Author(s): Imai H, Kubo S, Sasaki J, Shintani S
  • Journal Title: Three case reports., Ped. Dent. J Volume: 22(1) Pages: 84-89
  • Peer Reviewed
• [Journal Article] 知っておいて頂きたい小児歯科 (2012)
  • Author(s): 新谷誠康
  • Journal Title: 千葉小児科医会雑誌 Volume: 43 Pages: 3-8
• [Journal Article] う蝕と見誤っていませんか?見えてきたエナメル質形成不全症の実態 (2012)
  • Author(s): 新谷誠康
  • Journal Title: 歯科衛生士 Volume: 36(12) Pages: 56-6
• [Journal Article] 歯質の形成異常(日本小児歯科学会50周年記念特集小児歯科研究最前線) (2012)
  • Author(s): 新谷誠康
  • Journal Title: 小児歯誌(創立50周年記念誌) Pages: 48-49
• [Journal Article] 歯質の形成異常(日本小児歯科学会50周年記念特集小児歯科研究最前線) (2012)
  • Author(s): 新谷誠康
  • Journal Title: 小児歯科臨床 Volume: 17(9) Pages: 18-24
• [Journal Article] 口腔細菌に対する宿主免疫応答と疾患への関与 (2012)
  • Author(s): 桜井敦朗,新谷誠康
  • Journal Title: 小児歯誌 Volume: 50(1) Pages: 22-30
• [Presentation] 両生類Xenopus laevis(アフリカツメガエル)におけるDMP1遺伝子の同定および発現解析 (2013)
  • Author(s): 米倉智子、本間宏実、桜井敦朗、森口美津子、見明康雄、豊澤悟、新谷誠康
  • Organizer: 第51回日本小児歯科学会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-05-24
• [Presentation] Xenopus leavisの硬組織石灰化に関するDMP1の発現解析 (2012)
  • Author(s): 米倉智子、本間宏実、桜井敦朗、森口美津子、見明康雄、豊澤悟、新谷誠康
  • Organizer: 第294回東京歯科大学学会総会
  • Place of Presentation: 千葉-東京
  • Year and Date: 2012-10-20
• [Presentation] 両生類Xenopus laevis(アフリカツメガエル)におけるDMP1遺伝子の同定および発現解析 (2009)
  • Author(s): 米倉智子、本間宏実、桜井敦朗、森口美津子、見明康雄、豊澤悟、新谷誠康
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山
  • Year and Date: 2009-09-22
• [Book] 歯科臨床イヤーノート2014〜Section 8小児歯科5.高頻度治療 (2013)
  • Author(s): 新谷誠康(分担執筆),編集:住友雅人、木下淳博、沼部幸博、村松英雄
  • Total Pages: 603-608
  • Publisher: クインテッセンス出版株式会社,東京,分担
• [Book] 今日の小児治療指針第15版,小児歯科・口腔外科疾患歯周疾患 (2012)
  • Author(s): 新谷誠康(分担執筆),総編集:大関武彦,古川漸,横田俊一郎,水口雅
  • Total Pages: 855-857
  • Publisher: 医学書院,東京,分担
• [Remarks] 新谷誠康 エナメル質の形成障害 上海市口腔病防治院 上海市口腔医学会口腔予防専門部会 上海市予防医学会口腔衛生保健専門委員会等の教唆腕開催される国家継続医学教育の『口腔公共衛生活動の技術とその成績に関する評価法の講習会』 上海(中華人民共和国)2013.10.22
• [Remarks] 新谷誠康 エナメル質形成不全の実態とその治療教育講演 第32回日本小児歯科学会近畿地方会 奈良2013.9.29
• [Remarks] 新谷誠康 子どもの口腔ケア講演 千葉県医師会「子どもの生活習慣病研修」 千葉2013.3.23
• [Remarks] 新谷誠康 遺伝性エナメル質形成不全症の注意点と予防的治療講演 第2回臨床ゲノム医療学会 名古屋2012.12.16
• [Remarks] 新谷誠康 小児科医に知っておいていただきたい小児歯科講演 千葉県小児科医会7月例会 千葉2012.7.21
• [Remarks] 新谷誠康 歯の遺伝性障害,エナメル質形成不全症と象牙質形成不全症講演 第12回ゲノムドクター&キャスター東京セミナー 東京2012.7.8
• [Remarks] 新谷誠康 歯科医師の身近な先天異常-遺伝性エナメル質形成不全症講演,第293回東京歯科大学学会例会特別講演 千葉2012.6.2
• [Remarks] 新谷誠康 歯質の形成異常講演 第50回日本小児歯科学会記念大会リレー講演I「小児歯科研究最前線」 第50回日本小児歯科学会 東京2012.5.12(2012.5.12-5.13)