The autoantibody effects of periodontopathic bacteria on threatened preterm labor and preterm birth.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659921
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IZUMI Yuichi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60159803)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Hiroaki 東京医科歯科大学, 医歯学総合研究科, 助教 (50396967) ; TAKEUCHI Yasuo 東京医科歯科大学, 医歯学総合研究科, 助教 (60396968) ; KATAGIRI Sayaka 東京医科歯科大学, 医歯学総合研究科, 助教 (60510352)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 歯周病 / 早産 / 早期低体重児

Research Abstract

Periodontal disease is considered to be a risk factor for threatened preterm labor (TPL) and adverse delivery outcomes such as preterm birth and low birth weight (PLBW). Oral plaque and saliva were taken from 28 women diagnosed as TPL; a further 36 control samples were acquired from healthy pregnant women. Placenta samples were taken after delivery. Periodontopathic bacteria were identified by real-time PCR with TaqMan probe. Serum IgG antibodies against periodontopathic bacteria were measured by ELISA
Of 64 births, 13 delivered PLBW. Serum anti-P.gingivalis IgG antibody titer, plaque P.gingivalis levels, plaque T.forsythia level and saliva P.intermedia levels were significantly higher in the PLBW group compared with controls. Between TPL and healthy control group, there was no significant difference.
This study suggested that oral infection with P.gingivalis, T.forsythia and P.intermedia, and high level of anti-P.gingivalis IgG antibody titer are risk factors in PLBW.

Research Products

• [Journal Article] The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth (2013)
  • Author(s): Ye C, Katagiri S, Miyasaka N, Bharti P, Kobayashi H, Takeuchi Y, Momohara Y, Sekiguchi M, Takamine S, Nagasawa T, Izumi Y
  • Journal Title: Arch Gynecol Obstet Volume: 288(1) Pages: 65-72
  • DOI: 10.1007/s00404-013-2741-z
• [Journal Article] Application of quantitative proteomic analysis using tandem mass tags for discovery and identification of novel biomarkers in periodontal disease (2013)
  • Author(s): Tsuchida S, Satoh M, Kawashima Y, Sogawa K, Kado S, Sawai S, Nishimura M, Ogita M, Takeuchi Y, Kobyashi H, Aoki A, Kodera Y, Matsushita K, Izumi Y, Nomura F
  • Journal Title: Proteomics Volume: 13(15) Pages: 2339-50
  • DOI: 10.1002/pmic.201200510
• [Journal Article] Effect of glycemic control on periodontitis in type 2 diabetic patients with periodontal disease (2013)
  • Author(s): Katagiri S, Nitta H, Nagasawa T, Izumi Y, Kanazawa M, Matsuo A, Chiba H, Fukui M, Nakamura N, Oseko F, Kanamura N, Inagaki K, Noguchi T, Naruse K, Matsubara T, Miyazaki S, Miyauchi T, Ando Y, Hanada N, Inoue S
  • Journal Title: J Diabetes Investig Volume: 4(3) Pages: 320-325
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23997922
• [Presentation] 切迫早産と低体重児出産における歯周病原細菌とその抗体価に関する研究 (2014)
  • Author(s): 御給美沙、片桐さやか、小林宏明、竹内康雄、池田裕一、須田智也、須藤毅顕、和泉雄一
  • Organizer: 第140回日本歯科保存学会春季学術大会大津
  • Place of Presentation: 滋賀
  • Year and Date: 20140619-0620
• [Presentation] Periopathogenic bacteria in oral plaque, saliva and placenta from preterm low birth weight cases (2013)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 10th Asian Pacific Society of Periodontology meeting
  • Place of Presentation: Nara, Japan
  • Year and Date: 20130903-0904
• [Presentation] Periopathogenic Bacteria in Oral Plaque (2013)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: Saliva and Placenta from PLBW. International Academy of Dental Research
  • Place of Presentation: Seattle USA
  • Year and Date: 20130320-23
• [Presentation] The role of antibodies against periodontal pathogen on preterm birth (2012)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 98th Annual meeting American Academy of periodontology collaboration with the Japanese society of periodontology
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 20120929-1002